538299 M2I-1 - Calbiochem

538299
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
5.38299.0001
Retrieving availability...
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Glass bottle 10 mg
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity needs to be mulitiple of
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      Description
      OverviewA cell-permeable nitrobenzylidene-thiobarbiturate compound that directly targets the hydrophobic core of Mad2 and selectively disrupts Mad2-Cdc20 complex formation. Shown to dose-dependently inhibit the interaction of Mad2 to Mad2-binding-peptide (Mbp1; IC50 ~ 10.0 µM & Kd = 200 nM). Suggested to covalently modify Mad2-Cys149 via a reversible Michael adduct formation. Significantly reduces the mitotic duration in paclitaxel (0.5 µM; Cat. No. 580555)-treated HeLa cells (25 µM of M2I-1) and impairs mitotic spindle assembly checkpoint (SAC) function.

      Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
      Catalogue Number538299
      Brand Family Calbiochem®
      Synonyms5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione, 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione, Mad2 Inhibitor-1
      DescriptionM2I-1
      References
      ReferencesKastl, J., et al. 2015. ACS Chem. Biol. 10, 1661.
      Product Information
      FormOrange solid
      Hill FormulaC₁₉H₂₄N₄O₄S
      Chemical formulaC₁₉H₂₄N₄O₄S
      Hygroscopic Hygroscopic
      ReversibleY
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetMad2
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      M2I-1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Kastl, J., et al. 2015. ACS Chem. Biol. 10, 1661.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-September-2016 JSW
      Synonyms5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione, 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione, Mad2 Inhibitor-1
      DescriptionA cell-permeable nitrobenzylidene-thiobarbiturate compound that directly targets the hydrophobic core of Mad2 and selectively disrupts Mad2-Cdc20 complex formation. Shown to dose-dependently inhibit the interaction of Mad2 to Mad2-binding-peptide (Mbp1; IC50 ~ 10.0 µM & Kd = 200 nM). Suggested to covalently modify Mad2-Cys149 via a reversible Michael adduct formation. Significantly reduces the mitotic duration in paclitaxel (0.5 µM; Cat. No. 580555)-treated HeLa cells (25 µM of M2I-1) and impairs mitotic spindle assembly checkpoint (SAC) function.
      FormOrange solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₉H₂₄N₄O₄S
      Purity≥98% by HPLC
      SolubilityDMSO (50 mg/ml). Use only fresh DMSO for reconstitution.
      Storage +2°C to +8°C
      Hygroscopic
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Standard Handling
      ReferencesKastl, J., et al. 2015. ACS Chem. Biol. 10, 1661.