538299 Sigma-AldrichM2I-1 - Calbiochem
A cell-potent Mad2 selective modifier that disrupts Mad2-Cdc20 complex formation. Impairs cellular spindle assembly checkpoint function and significantly decreases mitotic duration.
More>> A cell-potent Mad2 selective modifier that disrupts Mad2-Cdc20 complex formation. Impairs cellular spindle assembly checkpoint function and significantly decreases mitotic duration. Less<<M2I-1 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Synonyms: 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione, 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione, Mad2 Inhibitor-1
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Overview
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Pricing & Availability
| Catalog Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
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| 5.38299.0001 |
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Glass bottle | 10 mg |
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| Description | |
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| Overview | A cell-permeable nitrobenzylidene-thiobarbiturate compound that directly targets the hydrophobic core of Mad2 and selectively disrupts Mad2-Cdc20 complex formation. Shown to dose-dependently inhibit the interaction of Mad2 to Mad2-binding-peptide (Mbp1; IC50 ~ 10.0 µM & Kd = 200 nM). Suggested to covalently modify Mad2-Cys149 via a reversible Michael adduct formation. Significantly reduces the mitotic duration in paclitaxel (0.5 µM; Cat. No. 580555)-treated HeLa cells (25 µM of M2I-1) and impairs mitotic spindle assembly checkpoint (SAC) function. Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water. |
| Catalogue Number | 538299 |
| Brand Family | Calbiochem® |
| Synonyms | 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione, 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione, Mad2 Inhibitor-1 |
| Description | M2I-1 |
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| References | Kastl, J., et al. 2015. ACS Chem. Biol. 10, 1661. |
| Product Information | |
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| Form | Orange solid |
| Hill Formula | C₁₉H₂₄N₄O₄S |
| Chemical formula | C₁₉H₂₄N₄O₄S |
| Hygroscopic | Hygroscopic |
| Reversible | Y |
| Quality Level | MQ100 |
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| Primary Target | Mad2 |
| Purity | ≥98% by HPLC |
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| Cell permeable | Y |
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| Packaged under inert gas | Packaged under inert gas |
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Documentation
M2I-1 - Calbiochem SDS
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References
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| Kastl, J., et al. 2015. ACS Chem. Biol. 10, 1661. |
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Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
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