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101287 Löffler's methylene blue solution

for microscopy

Löffler's methylene blue solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Löfflere'smethylene blue solution, Methylene blue solution acc. to Löffler

101287
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Overview

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      		 Glass bottle 100 ml
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      1012870500
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          		 Plastic bottle 500 ml
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          1012872500
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              		 Plastic bottle 2.5 l
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              Description
              Catalogue Number101287
              SynonymsLöfflere'smethylene blue solution, Methylene blue solution acc. to Löffler
              DescriptionLöffler's methylene blue solution
              OverviewLöffler’s methylene blue solution - for microscopy, is a ready-to-use-solution and is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use methylene blue is used for counterstaining.
              Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material.
              The 500 ml bottle is sufficient for approx. 250 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
              References
              Product Information
              HS Code3204 13 00
              Quality LevelMQ400
              Applications
              Applicationfor microscopy
              Biological Information
              Physicochemical Information
              Density0.97 g/cm3 (20 °C)
              Flash point40 °C
              pH value7.0 - 7.5 (H₂O, 20 °C)
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Hazard Pictogram(s) Hazard Pictogram(s)
              Hazard Statement(s)H226: Flammable liquid and vapour.
              Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
              P233: Keep container tightly closed.
              P240: Ground and bond container and receiving equipment.
              P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
              P242: Use non-sparking tools.
              P243: Take action to prevent static discharges.
              Signal WordWarning
              Storage class3 Flammable liquids
              WGKWGK 1 slightly hazardous to water
              Disposal3
              Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
              Safety Information
              Categories of dangerflammable
              Product Usage Statements
              Storage and Shipping Information
              StorageStore at +15°C to +25°C.
              Packaging Information
              Transport Information
              Declaration (railroad and road) ADR, RIDUN 1993 , 3, III
              Declaration (transport by air) IATA-DGRUN 1993 , 3, III
              Declaration (transport by sea) IMDG-CodeUN 1993 , 3, III
              Supplemental Information
              Specifications

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