LIF1010 Leukemia Inhibitory Factor human

LIF1010
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      CULT Human LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography. Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.
      Description
      Catalogue NumberLIF1010
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionLeukemia Inhibitory Factor human
      OverviewHuman LIF is a 19.7 kDa protein containing 181 amino acid residues. The non-glycosylated, E. coli expressed, recombinant human LIF is indistinguishable from native LIF in its biological activities in vitro. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid control. Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells, than hLIF.
      Alternate Names
      • LIF
      Background InformationLeukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.
      References
      Product Information
      PresentationLiquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.
      Quality LevelMQ300
      Applications
      Application10 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture
      Key Applications
      • Cell Culture
      Biological Information
      Concentration10 μg/mL
      PurityGreater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.
      SourceLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.
      Specific ActivityThe activity of human LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of human LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(E8) units/mg, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
      Entrez Gene Number
      Entrez Gene SummaryLeukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
      Gene Symbol
      • LIF
      • Emfilermin
      • MLPLI
      • HILDA
      • D-FACTOR
      • CDF
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
      SIZE: 202 amino acids; 22008 Da
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Mesenchymal Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY

        Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.

        The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.

        For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for 6 - 12 months. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added.



      Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Leukemia Inhibitory Factor human SDS

      Title

      Safety Data Sheet (SDS) 

      Leukemia Inhibitory Factor human Certificates of Analysis

      TitleLot Number
      Recombinant Human Leukemia Inhibitory Factor3066167
      Recombinant Human Leukemia Inhibitory Factor - 23188792318879
      Recombinant Human Leukemia Inhibitory Factor - 23942472394247
      Recombinant Human Leukemia Inhibitory Factor - 21236892123689
      Recombinant Human -24940402494040
      Recombinant Human -25301012530101
      Recombinant Human -25640152564015
      Recombinant Human -2581747A2581747A
      Recombinant Human -25818652581865
      Recombinant Human -25901502590150

      References

      Reference overviewPub Med ID
      Leukemia inhibitory factor, glial cell line-derived neurotrophic factor, and their receptor expressions following muscle crush injury.
      Kami, K, et al.
      Muscle Nerve, 22: 1576-86 (1999)  1999

      Show Abstract
      10514237 10514237
      In vitro expansion of a multipotent population of human neural progenitor cells.
      Carpenter, M K, et al.
      Exp. Neurol., 158: 265-78 (1999)  1999

      Show Abstract
      10415135 10415135

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors