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509306 Late Endosome Trafficking Inhibitor, EGA - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₆H₁₆BrN₃O

Products

Catalog NumberPackaging Qty/Pack
5.09306.0001 Glass bottle 25 mg
Description
OverviewA cell-permeable semicabazone compound that prevents B. anthrax Lethal toxin/LT (EC50 = 1.7 µM in RAW264.7 cultures; ECmax = 6 µM in murine BMDM cultures), diphtheria toxin/DT, P. aeruginosa ExoA, and H. ducreyi cytolethal distending toxin (Hd-CDT) from exerting their cytotoxic activity, but not endocytic uptake, by blocking endosomal trafficking/maturation into acidified late endosomes, while exhibiting no cytoprotective efficacy against ricin & E. coli Ec-CDT that do not require acidified endosomes for intoxication. Likewise, EGA is also shown to inhibit low-pH-dependent viral infectivity by Armstrong strain of LCMV, A/WSN/33 strain of influenza virus, but not low-pH-independent infection of Nipah virus NiV or MLV. Although the exact molecular target remains unknown, EGA does not act as a pH neutralizing agent, nor does it inhibit endosomal recycling or trafficking to phagocytic compartments.
Catalogue Number509306
Brand Family Calbiochem®
Synonyms4-Bromobenzaldehyde-N-(2,6-dimethylphenyl)semicarbazone, (E)-2-(4-Bromobenzylidene)-N-(2,6-dimethylphenyl)hydrazinecarboxamide
References
ReferencesGillespie, E.J., et al. 2013. Proc. Natl. Acad. Sci. USA 110, E4904.
Product Information
FormOff-white powder
Hill FormulaC₁₆H₁₆BrN₃O
Chemical formulaC₁₆H₁₆BrN₃O
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targethost-membrane trafficking
Purity≥93% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
5.09306.0001 04055977241822

Documentation

Late Endosome Trafficking Inhibitor, EGA - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Late Endosome Trafficking Inhibitor, EGA - Calbiochem Certificates of Analysis

TitleLot Number
509306

References

Reference overview
Gillespie, E.J., et al. 2013. Proc. Natl. Acad. Sci. USA 110, E4904.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-March-2014 JSW
Synonyms4-Bromobenzaldehyde-N-(2,6-dimethylphenyl)semicarbazone, (E)-2-(4-Bromobenzylidene)-N-(2,6-dimethylphenyl)hydrazinecarboxamide
DescriptionA cell-permeable semicarbazone compound that prevents B. anthrax Lethal toxin/LT (Protective Antigen/PA & LF/Lethal Factor complex; EC50 = 1.7 µM in RAW264.7 cultures; ECmax = 6 µM in murine BMDM cultures), diphtheria toxin (DT), LF-DT A chain fusion LFnDTA/PA complex, P. aeruginosa ExoA, and H. ducreyi cytolethal distending toxin (Hd-CDT) from exerting their cytotoxic activity, but not endocytic uptake, by blocking endosomal trafficking/maturation into acidified late endosomes, while exhibiting no cytoprotective efficacy against toxins that do not require acidified endosomes for intoxication, such as ricin & E. coli Ec-CDT. Likewise, EGA is also shown to inhibit low-pH-dependent viral infectivity by Armstrong strain of LCMV, A/WSN/33 strain of influenza virus, vesicular stomatitis virus VSV-pseudotyped lentivirus & VSV-pseudotyped MLV, but not low-pH-independent infection of Nipah virus NiV or MLV. Although the exact molecular target remains unknown, EGA does not act as a pH neutralizing agent, nor does it inhibit endosomal recycling or trafficking to phagocytic compartments.
FormOff-white powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₆H₁₆BrN₃O
Structure formulaStructure formula
Purity≥93% by HPLC
SolubilityDMSO (5 mg/ml). Use only fresh DMSO for reconstitution.
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesGillespie, E.J., et al. 2013. Proc. Natl. Acad. Sci. USA 110, E4904.