Pricing & Availability
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|71842-3||Plastic ampoule||100 rxn||
|71842-4||Glass bottle||500 rxn||
|Overview||PCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies. Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. EMD Millipore's molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility.
KOD Hot Start Master Mix* is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4. The Master Mix simplifies PCR set-up, offering time savings, consistency, and minimal risk of contamination. The mix is ideal for use in high-throughput applications. Simply add KOD Hot Start Master Mix to an equal volume of sample containing DNA template and primers. The final diluted reaction contains 1 U KOD Hot Start DNA Polymerase per 50 µl reaction. The smaller available size provides sufficient master mix for 100 (50 µl scale), or 250 (20 µl scale) reactions, while the larger size is adequate for 500 (50 µl scale) or 1250 (20 µl scale) reactions.
Features and Benefits:
|Unit of Definition||One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [<Sup>3</Sup>H]dTTP), and 150 µg/ml activated calf thymus DNA.|
|2 × 1.25 ml or 10 × 1.25 ml||KOD Hot Start Master Mix|
Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol. 289, 835.
|Declaration||* Manufactured by Toyobo and distributed by EMD. Not available from in Japan. Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.|
|Safety Information according to GHS|
|Product Usage Statements|
|Storage and Shipping Information|
|Ship Code||Shipped with Blue Ice or with Dry Ice|
|Do not freeze||Ok to freeze|
KOD Hot Start Master Mix SDS
|High fidelity gene amplification|
|PCR Protocols and Guides|
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