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IPSN08132 Immobilon®-P Blotting Sandwich

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      Key Specifications Table

      Key Applications
      Catalogue NumberIPSN08132
      Trade Name
      • Immobilon
      DescriptionImmobilon®-P Blotting Sandwich
      Background InformationOverview
      - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
      Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

      Immobilon-P Transfer Membrane:
      - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
      - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

      Blotting Sandwiches:
      - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

      Blotting Filter Paper:
      - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

      Immobilon-PSQ Membrane:
      - 0.2 µm pore size and a large internal structure
      - Higher protein adsorption and sequencing yields than other membranes
      - Recommended for blotting proteins less than 20 kDa
      - Prevents blow-through of low molecular weight proteins

      Immobilon-FL Membrane:
      - The first transfer membrane optimized for fluorescence applications
      - Extremely low background improves sensitivity of all fluorescence detection protocols
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      - Ideal for multiplexing and chemifluorescence applications

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

      Features & Benefits:
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be reprobed multiple times

      - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
      - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      Product Information
      Detection methodChemiluminescence Chromogenic Radioactive
      Filter CodeIPSN
      Filter ColorWhite
      Quality LevelMQ400
      Application8.5 x 13.5 cm, Immobilon-P membrane interleaved with blotting paper,for standard tank transfer procedures
      Key Applications
      • Western Blotting
      Biological Information
      BSA Adsorption215 µg/cm²
      Goat IgG Adsorption294 µg/cm²
      Insulin Adsorption160 µg/cm²
      Physicochemical Information
      Pore Size0.45 µm
      Filter SurfacePlain
      Filter Dimensions8.5 cm x 13.5 cm
      Materials Information
      • Hydrophobic Polyvinylidene Fluoride (PVDF)
      • Paper
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Material Size20
      Transport Information
      Supplemental Information