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405210 ID-8 - Calbiochem

405210
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405210-10MG
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      Glass bottle 10 mg
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      Description
      OverviewA cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.
      Catalogue Number405210
      Brand Family Calbiochem®
      Synonyms1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol
      References
      ReferencesMiyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem. 72, 1242.
      Product Information
      FormYellow powder
      Hill FormulaC₁₆H₁₄N₂O₄
      Chemical formulaC₁₆H₁₄N₂O₄
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ID-8 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      ID-8 - Calbiochem Certificates of Analysis

      TitleLot Number
      405210

      References

      Reference overview
      Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem. 72, 1242.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-October-2011 RFH
      Synonyms1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol
      DescriptionA cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.
      FormYellow powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₆H₁₄N₂O₄
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (50 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesMiyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem. 72, 1242.