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534331 Hypochlorous Acid Fluorescent Probe, PIS

A cell-permeable, selective & sensitive fluoro probe for HOCl. Enables HOCl detection by two-photon fluorescence microscopy in live cells & tissues.

Hypochlorous Acid Fluorescent Probe, PIS MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 1,3-Dimethyl-1H-imidazo[4,5-b]phenazine-2(3H)-thione, Hypochlorite Fluorescent Probe, MPO Activity Probe

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534331
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₅H₁₂N₄S

Products

Catalog NumberPackaging Qty/Pack
5.34331.0001 Glass bottle 10 mg
Description
OverviewA cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO·, ROO·, ONOO− and HO·). Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Also enables to monitor HOCl levels by two-photon image in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm) in a dose-dependent manner.
Catalogue Number534331
Brand Family Calbiochem®
Synonyms1,3-Dimethyl-1H-imidazo[4,5-b]phenazine-2(3H)-thione, Hypochlorite Fluorescent Probe, MPO Activity Probe
DescriptionHypochlorous Acid Fluorescent Probe, PIS
References
ReferencesXu, Q., et al. 2015. Angew. Chem. Int. Ed. 54, 4890.
Product Information
FormBrown solid
Hill FormulaC₁₅H₁₂N₄S
Chemical formulaC₁₅H₁₂N₄S
Quality LevelMQ100
Applications
Biological Information
Primary Targethypochlorous acid
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Identification Number
Catalog Number GTIN
5.34331.00014054839059360

Documentation

Hypochlorous Acid Fluorescent Probe, PIS SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Xu, Q., et al. 2015. Angew. Chem. Int. Ed. 54, 4890.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-June-2016 JSW
Synonyms1,3-Dimethyl-1H-imidazo[4,5-b]phenazine-2(3H)-thione, Hypochlorite Fluorescent Probe, MPO Activity Probe
DescriptionA cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO•, ROO•, ONOO- and HO•. Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Dose-dependently images HOCl levels by two-photon fluorescence microscopy in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm).
FormBrown solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₅H₁₂N₄S
Purity≥98% by HPLC
SolubilityDMSO (0.5 mg/ml) or DMF (0.5 mg/ml). Small yellow particles may be visible.
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesXu, Q., et al. 2015. Angew. Chem. Int. Ed. 54, 4890.