500511 Sigma-AldrichHistone Acetyltransferase Inhibitor VIII, NU9056 - Calbiochem

Safety Data Sheet (SDS) 

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	30-August-2013 JSW
Synonyms	1,2-Bis(isothiazol-5-yl)disulfane, 1,2-di(isothiazol-5-yl)disulfane, KAT5 Inhibitor I, Tip60 Inhibitor I, HAT Inhibitor VIII, p300/CBP Inhibitor VII, PCAF Inhibitor V
Description	A cell-permeable disulfane compound that is reported to be more potent against KAT5/Tip60 than PCAF, p300, and GCN5 in cell-free HAT activity assays (IC50 ≤2, ≤36, ≤60, and >100 µM, respectively) and effectively reduce H3K14, H4K8, H4K16, but not α-tubulin, acetylation level in LNCaP prostate cancer cells (max inhibition at 5-10 µM; 2 µM TSA, Cat. No. 647925, added in the last 4 h of 6 h NU9056 treatment to block HDAC activity). Shown to induce cell cycle arrest (% subG1 population after 24 h/48 h/72 h/ 96 h/dose = 9.3%/15%/22%/61%/17 µM and 12%/23%/25%/92%/24 µM) and apoptosis (fold caspase-9 cleavage increase/dose in 24 h = 2.5/17 µM & 3.5/24 µM; fold caspase-3 cleavage increase/dose in 96 h = 6.3/17 µM & 11/24 µM) in LNCaP cultures and exhibit antiproliferation activity in both androgen-responsive and androgen-independent CaP cultures (GI50 in three doubling time = 7.5, 12, 16, 24, and 27 µM, respectively, against WR22rvl, LNCaP-CdxR, LNCaP-AI, LNCaP, and PC3 cells).
Form	Yellowish-brown oil
Chemical formula	C₆H₄N₂S₄
Structure formula
Purity	≥97% by HPLC
Solubility	DMSO (50 mg/ml)
Storage	+2°C to +8°C Protect from light
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity	Standard Handling
References	Coffey, K., et al. 2012. PLoS One. 7, e45539.