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70796 His•Tag® Monoclonal Antibody


Replacement Information


Catalog NumberPackaging Qty/Pack
70796-3 Plastic ampoule 100 μg
70796-4 Plastic ampoule 3 μg

The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of the Novagen® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) enables sensitive, specific detection of His•Tag fusion proteins at antibody concentrations of 0.1-0.2 µg/ml. The His•Tag Monoclonal Antibody binds to N-terminal, C-terminal, and internal His•Tag sequences. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-µg package size provides enough purified antibody for 1000 ml of working solution.

* Not recommended for HRP-based detection of Trail Mix Western Markers.

Catalogue Number70796
Brand Family Novagen®
Product Information
Quality LevelMQ100
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Identification Number
Catalog Number GTIN


His•Tag® Monoclonal Antibody SDS


Safety Data Sheet (SDS) 

His•Tag® Monoclonal Antibody Certificates of Analysis

TitleLot Number


Protein Purification and Detection Tools


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  • User Protocols

    TB283 His•Tag® Monoclonal Antibody