01-201 Heregulin-β3, Epidermal growth factor (EGF) domain

01-201
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSource
      CULT Thrombin cleaved GST-fusion protein corresponding to the EGF domain (residues 178-241) of heregulin-beta 3.
      Description
      Catalogue Number01-201
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHeregulin-β3, Epidermal growth factor (EGF) domain
      References
      Product Information
      PresentationPBS with 30% glycerol. Liquid.
      Quality LevelMQ100
      Applications
      ApplicationThe Heregulin-β3 protein, Epidermal growth factor (EGF) domain is available in a 100 µg format.
      Key Applications
      • Cell Culture
      Biological Information
      SourceThrombin cleaved GST-fusion protein corresponding to the EGF domain (residues 178-241) of heregulin-beta 3.
      Entrez Gene Number
      Entrez Gene SummaryNeuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.
      Gene Symbol
      • NRG1
      • NDF
      • HRG
      • Pro-NRG1
      • HRGA
      • HRG1
      • SMDF
      • GGF2
      • HGL
      • ARIA
      • GGF
      Purification MethodSterilized through a 0.2 micron membrane filter
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15491 # The isoform SMDF may play a role in motor and sensory neuron development.| Q02297 # Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vessicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart.
      SIZE: 296 amino acids; 31686 Da
      SUBCELLULAR LOCATION: Secreted. Note=May possess an internal uncleaved signal sequence.
      TISSUE SPECIFICITY: Expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney.DEVELOPMENTAL STAGE: Highly expressed in developing spinal motor neurons and in developing cranial nerve nuclei. Expression is maintained only in both adult motor neurons and dorsal root ganglion neurons.
      DOMAIN: SwissProt: Q15491
      DISEASE: SwissProt: Q02297 # A rare chromosomal aberration involving NRG1 produces gamma-heregulin. Translocation t(8;11) with ODZ4. The translocation fuses the 5'-end of ODZ4 to NRG1 (isoform 8). The product of this translocation was first thought to be an alternatively spliced isoform. Gamma-heregulin is a soluble activating ligand for the ERBB2-ERBB3 receptor complex and acts as an autocrine growth factor in a specific breast cancer cell line (MDA-MB-175). Not detected in breast carcinoma samples, including ductal, lobular, medullary, and mucinous histological types, neither in other breast cancer cell lines.
      SIMILARITY: SwissProt: Q15491 ## Belongs to the neuregulin family. & Contains 1 EGF-like domain. | Q02297 # The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization (By similarity). & ERBB receptor binding is elicited entirely by the EGF-like domain.# Belongs to the neuregulin family. & Contains 1 EGF-like domain. & Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Stem Cell Type
      • Mesenchymal Stem Cells
      • Cardiac Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Receptor Activation Analysis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Heregulin-β3, Epidermal growth factor (EGF) domain SDS

      Title

      Safety Data Sheet (SDS) 

      Heregulin-β3, Epidermal growth factor (EGF) domain Certificates of Analysis

      TitleLot Number
      Heregulin-#946;, EGF Domain - DAM1776536DAM1776536
      Heregulin-#946;, EGF Domain - JBC1945024JBC1945024
      Heregulin-B, EGF Domain (recombinant protein expressed in E. coli)2816931
      Heregulin-b, EGF Domain - 21473222147322
      Heregulin-b, EGF Domain - DAM1588135DAM1588135
      Heregulin-b, EGF Domain - DAM1612113DAM1612113
      Heregulin-b, EGF Domain - DAM1647819DAM1647819
      Heregulin-beta;, EGF Domain - 1793217932
      Heregulin-beta;, EGF Domain - 2082520825
      Heregulin-beta;, EGF Domain - 2101321013

      References

      Reference overviewPub Med ID
      ErbB2 expression increases the spectrum and potency of ligand-mediated signal transduction through ErbB4.
      Wang, L M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 95: 6809-14 (1998)  1998

      Show Abstract
      9618494 9618494
      Epidermal growth factor and betacellulin mediate signal transduction through co-expressed ErbB2 and ErbB3 receptors.
      Alimandi, M, et al.
      EMBO J., 16: 5608-17 (1997)  1997

      9312020 9312020

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors