F0723-BC Ham's F-10 Liquid Medium with 10 mM HEPES

with 1.2 g/l NaHCO₃, without phenol red

Ham's F-10 Liquid Medium with 10 mM HEPES MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
F0723-BC
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      Overview

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      F0723
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          		 100 ml
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          Description
          OverviewNutrient mixture Ham's F-10 and F-12 were optimized for the serum free growth of hamster ovary strains and the clonal growth of chick embryo cells. Ham's F-10 is well suited for a wide variety of mammalian cells when used with a serum supplement in a 5% CO2 atmosphere. Ham's F-12 has higher levels of amino acids, vitamins and trace elements vs. Ham's F-10. Putrescine and linoleic acid were added to the formulation. Ham's F-12 today is the medium of choice for clonal toxicity-assays using CHO cells (CHD-3 and CHL-1).
          Specifications:

          Sterility: Pass
          BHK 21 Cell Proliferation Test: Pass
          BHK 21 Cell Cloning Efficicency Test: Pass
          pH: 7.2-7.6
          Osmolality: 0.27-0.30 Osm/kg H20
          Endotoxin: < 0.5 EU/ml
          Color: Light Pink
          Appearance: Clear
          Catalogue NumberF0723-BC
          Brand Family Calbiochem®
          References
          ReferencesHam. R.G. 1963. Exp. Cell Res. 29, 515.
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          Ship Code Blue Ice Only
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
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          Reference overview
          Ham. R.G. 1963. Exp. Cell Res. 29, 515.