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71011 HT96™ NovaBlue Competent Cells - Novagen

71011
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
71011-3 Glass bottle 1 plat
71011-4 Glass bottle 4 plat
Description
OverviewThe HT96™ NovaBlue Competent Cells are designed for high-throughput transformation. The cells are predispensed in 20-µl volumes in a sturdy 96-well polypropylene plate, which is compatible with a variety of thermal cyclers and water baths. Wells are individually sealed and have raised rims to prevent cross-contamination. Seals can be easily pierced with standard pipet tips or removed for access. Strips of caps are also provided for reliable sealing during manipulation and storage. Groups of 24 wells can be easily split from the plate for processing smaller numbers of samples.



HT96 NovaBlue Competent Cells
NovaBlue is a K-12 strain ideally suited as an initial cloning host because it has high transformation efficiency, blue/white screening capability (with appropriate plasmids), and mutations in recA and endA, which result in high yields of high-quality plasmid DNA.

Genotype:

endA1 hsdR17 (rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)


This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number71011
Brand Family Novagen®
Features and benefits• High-throughput, 96-well format
References
ReferencesPhillips, T.A., et al. 1984. J. Bacteriol. 159, 283.
Product Information
1 plate4 plates
1 plate4 platesHT96 NovaBlue Competent Cells
1 × 14 ml4 × 14 mlSOC Medium
1 × 10 µl2 × 10 µlTest Plasmid
1 pkg4 pkg8-Cap Strips
14Reagent Reservoirs
GenotypeendA1 hsdR17 (rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac Fʹ[proA+B+ lacIqZΔM15::Tn10] (TetR)
Guaranteed efficiency> 1 × 10⁸ cfu/µg
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
71011-3 04055977255713
71011-4 04055977255720

Documentation

HT96™ NovaBlue Competent Cells - Novagen SDS

Title

Safety Data Sheet (SDS) 

HT96™ NovaBlue Competent Cells - Novagen Certificates of Analysis

TitleLot Number
71011

References

Reference overview
Phillips, T.A., et al. 1984. J. Bacteriol. 159, 283.

Brochure

Title
Express yourself
High fidelity gene amplification
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

Citations

Title
  • Jonathan Caruthers, et al. (2005) Crystal structures and proposed structural/functional classification of three protozoan proteins from the isochorismatase superfamily. Protein Science 14, 2887-2894.