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F0483-BC HAT-Supplement

F0483-BC
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F0483
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      100 ml
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      Description
      OverviewHAT Supplement is a liquid mixture of sodium hypoxanthine, aminopterin and thymidine. After dilution, HAT is suitable for use as a post-fusion selective medium to eliminate unfused or self-fused HGPRT-myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway.

      Specifications:

      Sterility: Pass
      pH: 9.0-11
      Osmolality: 0.265-0.295 Osm/kg H20
      Color: Colorless
      Appearance: Clear

      Catalogue NumberF0483-BC
      Brand Family Calbiochem®
      References
      Product Information
      Quality LevelMQ300
      Applications
      Biological Information
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      Storage and Shipping Information
      Ship Code Dry Ice Only
      Storage -20°C
      Do not freeze Ok to freeze
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