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371958 H-8, Dihydrochloride

A potent, cell-permeable, reversible, and ATP-competitive inhibitor of cyclic-nucleotide-dependent protein kinases.

H-8, Dihydrochloride MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-[2-(Methylamino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor I

Primary Target: MLCK
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
113276-94-1C₁₂H₁₅N₃O₂S · 2HCl

Products

Catalog NumberPackaging Qty/Pack
371958-1MG Plastic ampoule 1 mg
Description
OverviewA potent, cell-permeable, reversible, and ATP-competitive inhibitor of cyclic-nucleotide-dependent protein kinases. Inhibits protein kinase A (Ki = 1.2 µM), myosin light chain kinase (Ki = 68 µM), protein kinase C (Ki = 15 µM), and protein kinase G (Ki = 480 nM).
Catalogue Number371958
Brand Family Calbiochem®
SynonymsN-[2-(Methylamino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor I
References
ReferencesIdo, M., et al. 1991. Br. J. Cancer 64, 1103.
Hagiwara, M., et al. 1987. Mol. Pharmacol. 31, 523.
Inagaki, M., et al. 1986. Mol. Pharmacol. 29, 577.
Hidaka, H., et al. 1984. Biochemistry 23, 5036.
Product Information
CAS number113276-94-1
ATP CompetitiveY
FormLyophilized
Hill FormulaC₁₂H₁₅N₃O₂S · 2HCl
Chemical formulaC₁₂H₁₅N₃O₂S · 2HCl
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetMLCK
Primary Target K<sub>i</sub>68 µM against MLCK
Purity>95% by TLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

H-8, Dihydrochloride SDS

Title

Safety Data Sheet (SDS) 

H-8, Dihydrochloride Certificates of Analysis

TitleLot Number
371958

References

Reference overview
Ido, M., et al. 1991. Br. J. Cancer 64, 1103.
Hagiwara, M., et al. 1987. Mol. Pharmacol. 31, 523.
Inagaki, M., et al. 1986. Mol. Pharmacol. 29, 577.
Hidaka, H., et al. 1984. Biochemistry 23, 5036.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-January-2010 RFH
SynonymsN-[2-(Methylamino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor I
DescriptionA potent, cell-permeable, reversible, and ATP-competitive, and highly active inhibitor of cyclic-nucleotide-dependent protein kinases. Inhibits myosin light chain kinase (Ki = 68 µM), protein kinase A (Ki = 1.2 µM), protein kinase C (Ki = 15 µM), and protein kinase G (Ki = 480 nM).
FormLyophilized
CAS number113276-94-1
Chemical formulaC₁₂H₁₅N₃O₂S · 2HCl
Structure formulaStructure formula
Purity>95% by TLC
SolubilityH₂O (10 mg/ml). Further dilute with aqueous buffer just prior to use.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C.
Toxicity Standard Handling
ReferencesIdo, M., et al. 1991. Br. J. Cancer 64, 1103.
Hagiwara, M., et al. 1987. Mol. Pharmacol. 31, 523.
Inagaki, M., et al. 1986. Mol. Pharmacol. 29, 577.
Hidaka, H., et al. 1984. Biochemistry 23, 5036.