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345852 Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

345852
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345852-50UG
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      Description
      OverviewRecombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ for optimal activity.
      Catalogue Number345852
      Brand Family Calbiochem®
      SynonymsGGTase I
      References
      ReferencesKalinin, A., et al. 2001. Protein Expr. Purif. 22, 84.
      Zhang, F.L., et al. 1994. J. Biol. Chem. 269, 23465.
      Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem. 267, 17438.
      Product Information
      FormLiquid
      FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
      Quality LevelMQ100
      Applications
      ApplicationGeranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli, contains a 74 kDa α- & 43 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag.
      Biological Information
      Biological activity1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      345852

      References

      Reference overview
      Kalinin, A., et al. 2001. Protein Expr. Purif. 22, 84.
      Zhang, F.L., et al. 1994. J. Biol. Chem. 269, 23465.
      Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem. 267, 17438.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-June-2008 RFH
      SynonymsGGTase I
      DescriptionRecombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved by TEV protease. Requires Zn2+ for optimal activity. The enzyme is purified with Zn2+ tightly bound, so addition of Zn2+ to the reaction mix is not necessary unless further manipulation of the enzyme results in release of the ions.
      FormLiquid
      FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
      Recommended reaction conditions50 mM HEPES, pH 7.2, 5 mM MgCl2, 1 mM DTT, 3 mM NP-40 alternative, 5.5 µM [3H]GG pyrophosphate, 30 pmol acceptor protein of choice, and desired amount of enzyme; incubate at 37°C for 20 min.; quench the reaction with 10% HCl in 90% Ethanol.
      Purity≥90% by SDS-PAGE
      Biological activity1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesKalinin, A., et al. 2001. Protein Expr. Purif. 22, 84.
      Zhang, F.L., et al. 1994. J. Biol. Chem. 269, 23465.
      Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem. 267, 17438.