371770 G-Protein Subunit Antibody Set

371770
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Overview

Replacement Information

Key Specifications Table

HostAntibody Type
Rb Polyclonal Antibody

Pricing & Availability

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371770-1ST
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      Description
      OverviewContains 10 µl of each of the following antibodies: Anti-Giα-1 (Cat. No. 371720), Anti-Giα-1 and Giα-2 (Cat. No. 371723), Anti-Giα-3 and Goα (Cat. No. 371726), Anti-Giα-3 (Cat. No. 371729), Anti-Gsα (Cat. No. 371732), Anti-Gq- and G11α (Cat. No. 371751), and Anti-Gβ-Subunit (Cat. No. 371738). Each antibody in the set is sufficient for one Western blot at 1:1000.
      See individual antibody descriptions for more details.
      Catalogue Number371770
      Brand Family Calbiochem®
      References
      ReferencesMumby, S., et al. 1988. J. Biol. Chem. 263, 2020.
      Yatani, A., et al. 1988. Nature 336, 680.
      Mumby, S.M., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 265.
      Product Information
      FormLiquid
      FormulationEach antibody is supplied in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
      PreservativeNone
      Quality LevelMQ100
      Applications
      ApplicationG-Protein Subunit Antibody Set. Contains 10 µl of each of Anti-Giα-1, Anti-Giα-1 and Giα-2, Anti-Giα-3 and Goα, Anti-Giα-3, Anti-Gsα, Anti-Gq- and G11α, and Anti-Gβ-Subunit.
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000, all antibodies)
      Application CommentsThe specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes encoding each subunit. For immunoblotting: 10 µg purified plasma membranes from rat adipocytes were loaded into each lane and resolved on a 12% acrylamide mini-gel (10 cm x 10 cm). The proteins were transferred to PVDF membranes on a semi-dry blotter and blocked in low detergent blotto (5% milk protein) for 1 hr. The blots were transferred to designated vessels containing the specific antibody diluted in low detergent blotto at 1:1000. Each blot was incubated on a rocker at room temperature with its specific antibody for 2 h, followed by 4 washes with low detergent blotto. Goat anti-rabbit IgG labeled with 125I (approximately 5µCi/µg IgG) was diluted to 500,000 cpm/ml in low detergent blotto, followed by several washes to remove milk proteins. The blots were exposed to X-ray film with intensifying screens for the indicated times. Antibodies should be titrated for optimal results in individual systems.
      Biological Information
      HostRabbit
      IsotypeIgG
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      G-Protein Subunit Antibody Set SDS

      Title

      Safety Data Sheet (SDS) 

      G-Protein Subunit Antibody Set Certificates of Analysis

      TitleLot Number
      371770

      References

      Reference overview
      Mumby, S., et al. 1988. J. Biol. Chem. 263, 2020.
      Yatani, A., et al. 1988. Nature 336, 680.
      Mumby, S.M., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 265.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      ApplicationImmunoblotting (1:1000, all antibodies)
      DescriptionAntibody set containing 10 µl of each of the following antibodies:

      Anti-Giα-1 (Cat. No. 371720)
      Anti-Giα-1 and Giα-2 (Cat. No. 371723)
      Anti-Giα-3 (Cat. No. 371729)
      Anti-Giα-3 and Goα (Cat. No. 371726)
      Anti-Gsα (Cat. No. 371732)
      Anti-G-protein β-subunit (Cat. No. 371738)
      Anti-Gqα and G11α (Cat. No. 371751)

      Useful for identifying G-protein subunits.
      BackgroundGTP-binding regulatory proteins (G-proteins) are heterotrimeric proteins associated with the cell membrane that functionally couple hormones to their receptor systems within the cell. These proteins link signaling pathways that regulate the synthesis of cyclic AMP, as well as other pathways, including activation of phospholipases and mobilization of intracellular calcium. The original term, inhibitory G-proteins (Gi), refers to a family of G-proteins distinguished by differences in the α-subunit of the heterotrimer. It is believed that the different Gi's serve to couple different hormone receptors to different effector systems. However, some G-proteins may not be discriminating because they appear to interact with more than one type of receptor or effector system.
      HostRabbit
      IsotypeIgG
      FormLiquid
      FormulationEach antibody is supplied in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
      PreservativeNone
      CommentsThe specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes encoding each subunit. For immunoblotting: 10 µg purified plasma membranes from rat adipocytes were loaded into each lane and resolved on a 12% acrylamide mini-gel (10 cm x 10 cm). The proteins were transferred to PVDF membranes on a semi-dry blotter and blocked in low detergent blotto (5% milk protein) for 1 hr. The blots were transferred to designated vessels containing the specific antibody diluted in low detergent blotto at 1:1000. Each blot was incubated on a rocker at room temperature with its specific antibody for 2 h, followed by 4 washes with low detergent blotto. Goat anti-rabbit IgG labeled with 125I (approximately 5µCi/µg IgG) was diluted to 500,000 cpm/ml in low detergent blotto, followed by several washes to remove milk proteins. The blots were exposed to X-ray film with intensifying screens for the indicated times. Antibodies should be titrated for optimal results in individual systems.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesMumby, S., et al. 1988. J. Biol. Chem. 263, 2020.
      Yatani, A., et al. 1988. Nature 336, 680.
      Mumby, S.M., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 265.