345810 G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

345810
View Pricing & Availability

Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₂₀H₄₀N₄O₁₀ · 2H₂SO₄ 108321-42-2

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
345810-100GM
Retrieving availability...
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Glass bottle 100 gm
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity needs to be mulitiple of
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      345810-1GM
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 1 gm
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          345810-250MG
          Retrieving availability...
          Limited AvailabilityLimited Availability
          Stocked 
          Discontinued
          Limited Quantities Available
          Available
            Remaining : Will advise
              Remaining : Will advise
              Will advise
              Contact Customer Service
              Contact Customer Service

              Plastic ampoule 250 mg
              Retrieving price...
              Price could not be retrieved
              Minimum Quantity needs to be mulitiple of
              Upon Order Completion More Information
              You Saved ()
               
              Request Pricing
              345810-25GM
              Retrieving availability...
              Limited AvailabilityLimited Availability
              Stocked 
              Discontinued
              Limited Quantities Available
              Available
                Remaining : Will advise
                  Remaining : Will advise
                  Will advise
                  Contact Customer Service
                  Contact Customer Service

                  Plastic ampoule 25 gm
                  Retrieving price...
                  Price could not be retrieved
                  Minimum Quantity needs to be mulitiple of
                  Upon Order Completion More Information
                  You Saved ()
                   
                  Request Pricing
                  345810-500MG
                  Retrieving availability...
                  Limited AvailabilityLimited Availability
                  Stocked 
                  Discontinued
                  Limited Quantities Available
                  Available
                    Remaining : Will advise
                      Remaining : Will advise
                      Will advise
                      Contact Customer Service
                      Contact Customer Service

                      Plastic ampoule 500 mg
                      Retrieving price...
                      Price could not be retrieved
                      Minimum Quantity needs to be mulitiple of
                      Upon Order Completion More Information
                      You Saved ()
                       
                      Request Pricing
                      345810-5GM
                      Retrieving availability...
                      Limited AvailabilityLimited Availability
                      Stocked 
                      Discontinued
                      Limited Quantities Available
                      Available
                        Remaining : Will advise
                          Remaining : Will advise
                          Will advise
                          Contact Customer Service
                          Contact Customer Service

                          Glass bottle 5 gm
                          Retrieving price...
                          Price could not be retrieved
                          Minimum Quantity needs to be mulitiple of
                          Upon Order Completion More Information
                          You Saved ()
                           
                          Request Pricing
                          Description
                          OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
                          Also available as a sterile-filtered aqueous solution (Cat. No. 345812), a sterile filtered solution in 100 mM HEPES (Cat. No. 508940), and a sterile-filtered solution PBS (Cat. No. 509227).
                          Catalogue Number345810
                          Brand Family Calbiochem®
                          SynonymsNMD Inhibitor I, G418, G 418
                          References
                          ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                          Jimenez, A., and Davies, J. 1980. Nature 287, 869.
                          Product Information
                          CAS number108321-42-2
                          FormWhite powder
                          Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                          Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                          Structure formula ImageStructure formula Image
                          Quality LevelMQ100
                          Applications
                          Biological Information
                          Biological activityAssay: ≥730 µg/mg
                          Purity≥98% by TLC
                          Physicochemical Information
                          ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
                          Dimensions
                          Materials Information
                          Toxicological Information
                          Safety Information according to GHS
                          RTECSCB9378500
                          Safety Information
                          Product Usage Statements
                          Storage and Shipping Information
                          Ship Code Ambient Temperature Only
                          Toxicity Standard Handling
                          Storage +15°C to +30°C
                          Do not freeze Ok to freeze
                          Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
                          Packaging Information
                          Transport Information
                          Supplemental Information
                          Specifications

                          Documentation

                          G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem SDS

                          Title

                          Safety Data Sheet (SDS) 

                          G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificates of Analysis

                          TitleLot Number
                          345810

                          References

                          Reference overview
                          Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                          Jimenez, A., and Davies, J. 1980. Nature 287, 869.

                          Brochure

                          Title
                          Antibiotics Profiler
                          Bulk packaging
                          G418/Hygromycin B Flyer
                          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (MilliporeSigma)

                          Technical Info

                          Title
                          Molecular Biology Grade Reagents

                          Citations

                          Title
                        • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
                        • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
                        • Data Sheet

                          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                          Revision01-December-2011 JSW
                          SynonymsNMD Inhibitor I, G418, G 418
                          DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
                          FormWhite powder
                          Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
                          CAS number108321-42-2
                          RTECSCB9378500
                          Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                          Structure formulaStructure formula
                          Purity≥98% by TLC
                          ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
                          Biological activityAssay: ≥730 µg/mg
                          SolubilitySolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
                          Storage +15°C to +30°C
                          Do Not Freeze Ok to freeze
                          Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
                          Toxicity Standard Handling
                          ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                          Jimenez, A., and Davies, J. 1980. Nature 287, 869.
                          Citation
                        • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
                        • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
                        • Related Products & Applications

                          Alternative Products

                          Catalog Number Description  
                          345812 G 418 Sulfate, Sterile-Filtered Aqueous Solution, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Show Pricing & Availability

                          Categories

                          Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Other Inhibitors of Biological Interest > Protein Synthesis Inhibitors
                          Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents
                          Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents