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345810 G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

345810
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Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₂₀H₄₀N₄O₁₀ · 2H₂SO₄ 108321-42-2

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345810-100GM
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      Glass bottle 100 gm
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      345810-250MG
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          Plastic ampoule 250 mg
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          345810-500MG
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              Description
              OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
              Also available as a sterile-filtered aqueous solution (Cat. No. 345812), a sterile filtered solution in 100 mM HEPES (Cat. No. 508940), and a sterile-filtered solution PBS (Cat. No. 509227).
              Catalogue Number345810
              Brand Family Calbiochem®
              SynonymsNMD Inhibitor I, G418, G 418
              References
              ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
              Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
              Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
              Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
              Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
              Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
              Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
              Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
              Jimenez, A., and Davies, J. 1980. Nature 287, 869.
              Product Information
              CAS number108321-42-2
              FormWhite powder
              Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
              Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
              Structure formula ImageStructure formula Image
              Quality LevelMQ100
              Applications
              Biological Information
              Biological activityAssay: ≥730 µg/mg
              Purity≥98% by TLC
              Physicochemical Information
              ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSCB9378500
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage +15°C to +30°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem SDS

              Title

              Safety Data Sheet (SDS) 

              G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificates of Analysis

              TitleLot Number
              345810

              References

              Reference overview
              Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
              Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
              Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
              Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
              Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
              Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
              Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
              Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
              Jimenez, A., and Davies, J. 1980. Nature 287, 869.

              Brochure

              Title
              Antibiotics Profiler
              Bulk packaging
              G418/Hygromycin B Flyer
              The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (MilliporeSigma)

              Technical Info

              Title
              Molecular Biology Grade Reagents

              Citations

              Title
            • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
            • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
            • Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision01-December-2011 JSW
              SynonymsNMD Inhibitor I, G418, G 418
              DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
              FormWhite powder
              Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
              CAS number108321-42-2
              RTECSCB9378500
              Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
              Structure formulaStructure formula
              Purity≥98% by TLC
              ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
              Biological activityAssay: ≥730 µg/mg
              SolubilitySolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
              Storage +15°C to +30°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
              Toxicity Standard Handling
              ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
              Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
              Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
              Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
              Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
              Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
              Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
              Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
              Jimenez, A., and Davies, J. 1980. Nature 287, 869.
              Citation
            • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
            • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.