GF003 Fibroblast Growth Factor basic Protein, Human recombinant

GF003
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      CULT, Cult Human expressed in E. coli Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 EU per μg of FGF-b.
      Description
      Catalogue NumberGF003
      ReplacesFA009-1
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionFibroblast Growth Factor basic Protein, Human recombinant
      OverviewFibroblast Growth Factor-basic (FGF-b) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in vivo. Human FGF-b is a 17.2 kDa protein containing 155 amino acid residues.
      Alternate Names
      • FGF-2
      • bFGF
      • beta-FGF
      • Heparin-Binding Growth Factor 2
      Background InformationFibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003-AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system.
      References
      Product Information
      PresentationLyophilized from a 0.7 mg/mL solution in 5mM Tris, pH 7.6 with 150 mM NaCl.
      Quality LevelMQ100
      Applications
      ApplicationThe Fibroblast Growth Factor-basic (FGF-b) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Application NotesFor most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/mL, depending on the method of culture.

      Optimal working dilutions must be determined by end user.
      Biological Information
      PurityGreater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 EU per μg of FGF-b.
      Sourceexpressed in E. coli
      Specific ActivityChemicon's Human FGF-b is fully biologically active when compared to standards. The ED<sub>50</sub> was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
      Gene Symbol
      • FGF2
      • prostatropin
      • Prostatropin
      • HBGF-2
      • BFGF
      • FGFB
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
      SIZE: 155 amino acids; 17254 Da
      SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2.
      SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family.
      MISCELLANEOUS: This protein binds heparin more strongly than does aFGF.
      Stem Cell Type
      • Neural Stem Cells
      • Epithelial Cells
      • Mesenchymal Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain the lyophilized material at -20°C until expiration date as stated on the label.

      General applications:
      After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20 °C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity.

      For Human ES cell culture:
      After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco's PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Fibroblast Growth Factor basic Protein, Human recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      Fibroblast Growth Factor basic Protein, Human recombinant Certificates of Analysis

      TitleLot Number
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC2462893
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC2975993
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC2842669
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC2872536
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC3070090
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 21396652139665
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 23847202384720
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC - Q2436472Q2436472
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 20131282013128
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 20477652047765

      Technical Info

      Title
      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Accumax Dissociation Reagent for the Serial Passaging of Human Embryonic Stem Cells
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

      Data Sheet

      Title
      RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR-BASIC

      Newsletters / Publications

      Title
      Cellutions Newsletter: 2007, Volume 1
      Cellutions Newsletter: 2008, Volume 1
      Cellutions Newsletter: 2008, Volume 3

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors