GF003-AF Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant

GF003-AF
50 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      CULT, Cult Human expressed in E. coli Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 EU per μg of bFGF.
      Description
      Catalogue NumberGF003-AF
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionFibroblast Growth Factor basic Protein, Human Animal-Free recombinant
      Alternate Names
      • FGF-2
      • bFGF
      • basic-FGF
      • Heparin-Binding Growth Factor 2
      • Prostatropin
      Background InformationFibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003-AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system.
      References
      Product Information
      PresentationLyophilized from a 0.1 mg/mL solution of 5mM Tris, pH 7.6 with 150 mM NaCl.
      Quality LevelMQ100
      Applications
      ApplicationThe Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Application NotesFor most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/ml, depending on the method of culture
      Biological Information
      PurityGreater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 EU per μg of bFGF.
      Sourceexpressed in E. coli
      Specific ActivityHuman FGF-b is fully biologically active when compared to standards. The ED<sub>50</sub> was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
      Gene Symbol
      • FGF2
      • prostatropin
      • Prostatropin
      • HBGF-2
      • BFGF
      • FGFB
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
      SIZE: 155 amino acids; 17254 Da
      SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2.
      SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family.
      MISCELLANEOUS: This protein binds heparin more strongly than does aFGF.
      Stem Cell Type
      • Neural Stem Cells
      • Epithelial Cells
      • Mesenchymal Stem Cells
      • Pancreatic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain the lyophilized material at -20°C until expiration date as stated on the label.

      General applications:
      After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity.

      For Human ES cell culture:
      After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco's PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      Fibroblast Growth Factor basic Protein, Human Animal-Free recombinant Certificates of Analysis

      TitleLot Number
      RECOMBINANT ANIMAL-FREE2470845
      RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC -28236232823623
      RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 23839722383972
      RECOMBINANT ANIMAL-FREE -25817412581741
      RECOMBINANT ANIMAL-FREE -25901542590154
      RECOMBINANT ANIMAL-FREE -25923172592317
      RECOMBINANT ANIMAL-FREE -26543122654312
      RECOMBINANT ANIMAL-FREE -27009612700961
      RECOMBINANT ANIMAL-FREE -27144272714427
      RECOMBINANT ANIMAL-FREE -27767152776715

      References

      Reference overviewPub Med ID
      Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells
      Xu, Ren-He, et al
      Nat Methods, 2:185-190 (2005)  2005

      15782187 15782187

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors