344045 FOCUS-Protease Arrest™ Cocktail - Calbiochem

A protease cocktail specifically developed for 2D gel sample preparation.

FOCUS-Protease Arrest™ Cocktail - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
344045
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      344045-1ML
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          		 Plastic ampoule 1 ml
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          Description
          OverviewA protease cocktail specifically developed for 2D gel sample preparation. Inhibits serine, cysteine, metalloproteinases, and calpain proteases from animal tissues, plants, yeast, and bacteria. FOCUS-Protease Arrest™ Cocktail at 1X concentration in extraction buffer, pH 7.0 - 8.0, inhibits 95-98% of protease activity.
          Catalogue Number344045
          Brand Family Calbiochem®
          References
          Product Information
          ATP CompetitiveN
          FormLiquid
          FormulationSupplied as 100X.
          ReversibleN
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetSerine, cysteine, metalloproteinases, and calpain proteases
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Irritant
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest™ inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          FOCUS-Protease Arrest™ Cocktail - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          FOCUS-Protease Arrest™ Cocktail - Calbiochem Certificates of Analysis

          TitleLot Number
          344045
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-September-2007 RFH
          DescriptionA protease inhibitor cocktail specifically developed for the inhibition of protease activity during protein purification and sample processing. Suitable for use with yeast, bacterial, plants, and mammalian tissues. No EDTA is used in this preparation. Compatible with FOCUS-Nuclease™ for removing nucleic acid contamination from samples. FOCUS-Protease-Arrest™ contains agents that inhibit serine, cysteine, metallo-proteases as well as calpains. FOCUS-Protease-Arrest™ contains both irreversible and reversible protease inhibitors. FOCUS-Protease Arrest™, used at 1:50 dilution in extraction buffer (pH 7-8) inhibits over 95-98% of protease activities.
          FormLiquid
          FormulationSupplied as 100X.
          Recommended reaction conditions
          1. Allow solution to warm to room temperature. The solution is in suspension form, vortex before pipetting. 2. Add FOCUS-Protease Arrest™ Cocktail at a volume of 10 µl/ml of extraction/lysis buffer or extract to achieve a 1X final concentration. Note: For greater protease inhibition, the Protease Arrest™ Cocktail may be added to extraction/lysis buffer at a volume of 13 µl/ml (1.3X final concentration).
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest™ inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.
          Toxicity Irritant