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343242 FLUO 3/AM - CAS 121714-22-5 - Calbiochem

Membrane-permeable version of FLUO 3. Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.

FLUO 3/AM - CAS 121714-22-5 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2ʹ-amino-5ʹ-methylphenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Pentaacetoxymethyl Ester

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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
121714-22-5C₅₁H₅₀Cl₂N₂O₂₃

Products

Catalog NumberPackaging Qty/Pack
343242-1MG Glass bottle 1 mg
Description
OverviewMembrane-permeable version of FLUO 3 (Cat. No. 343244). Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.
Catalogue Number343242
Brand Family Calbiochem®
Synonyms1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2ʹ-amino-5ʹ-methylphenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Pentaacetoxymethyl Ester
References
ReferencesCaputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.
Product Information
CAS number121714-22-5
ATP CompetitiveN
FormReddish orange solid
Hill FormulaC₅₁H₅₀Cl₂N₂O₂₃
Chemical formulaC₅₁H₅₀Cl₂N₂O₂₃
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetCa2+ indicators
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
343242-1MG 04055977195095

Documentation

FLUO 3/AM - CAS 121714-22-5 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

FLUO 3/AM - CAS 121714-22-5 - Calbiochem Certificates of Analysis

TitleLot Number
343242

References

Reference overview
Caputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.

Brochure

Title
Fluorescent Calcium Probes Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-December-2018 JSW
Synonyms1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2ʹ-amino-5ʹ-methylphenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Pentaacetoxymethyl Ester
DescriptionMembrane-permeable version of FLUO 3 (Cat. No. 343244). Cleavage of FLUO 3/AM by cytosolic esterases traps the indicator in the cell. FLUO 3 is almost nonfluorescent at resting calcium levels, but fluorescence levels increase 40-fold upon calcium binding. Unlike all other Ca2+ indicators, FLUO 3/AM is nonfluorescent until it is hydrolyzed by the cell. Since its excitation maximum is at 506 nm, this compound can be used in conjunction with caged compounds, which are released by UV light. Its relatively high Kd for Ca2+ facilitates the measurement of higher peaks of Ca2+ transients than is possible with FURA 2. FLUO 3 has an excitation maximum of 506 nm and an emission maximum of 526 nm in the presence of high and low calcium concentrations.
FormReddish orange solid
Recommended reaction conditionsThe FLUO 3/AM DMSO stock solution should be diluted to 1-5 µM in a physiological buffer. In some cases, use of the non-ionic detergent PLURIONIC® F-127, PROTEIN GRADE® (Cat. No. 540025) at 0.025-0.125% will assist in the dissolution of the dye in aqueous buffers. Cells are normally incubated with the dye for 20-60 min at 20-37°C.
CAS number121714-22-5
Chemical formulaC₅₁H₅₀Cl₂N₂O₂₃
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMF and DMSO (5 mM)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesCaputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.