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341590 FGF, Basic, Rat, Recombinant

FGF, Basic, Rat, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HBGF-2

341590
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Overview

Replacement Information

Key Specifications Table

Purity
≥95% by SDS-PAGE

Pricing & Availability

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341590-25UG
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      		 Glass bottle 25 μg
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      Description
      OverviewRecombinant, rat fibroblast growth factor (FGF) basic consisting of amino acids 11-154 and expressed in E. coli. FGF basic, a member of the FGF family of mitogenic peptides, is known to bind heparine or cell surface heparin sulfate proteoglycans for binding, dimerization, and activation of tyrosine kinase FGF receptors. Also known to bind polysaccharides, proteins, and lipids with lower affinity.
      Catalogue Number341590
      Brand Family Calbiochem®
      SynonymsHBGF-2
      References
      ReferencesPresta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
      Quarto, N., et al. 2005. Gene 356, 49.
      Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun. 335, 1239.
      Claus, P., et al. 2003. J. Biol. Chem. 278, 479.
      Coulier, F., et al. 1997. J. Mol. Evol. 44, 43.
      Fernig, D., et al. 1994. Prog. Growth Factor Res. 5, 353.
      Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun. 157, 256.
      Product Information
      CAS number7757-82-6, 1132-61-2, 60-00-4, 9048-46-8, 106096-93-9, 3483-12-3
      FormSolid
      FormulationLyophilized from 0.2 µm filtered MOPS, Na₂SO₄, EDTA, DTT, BSA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 0.3-1.8 ng/ml as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      FGF, Basic, Rat, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      FGF, Basic, Rat, Recombinant Certificates of Analysis

      TitleLot Number
      341590

      References

      Reference overview
      Presta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
      Quarto, N., et al. 2005. Gene 356, 49.
      Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun. 335, 1239.
      Claus, P., et al. 2003. J. Biol. Chem. 278, 479.
      Coulier, F., et al. 1997. J. Mol. Evol. 44, 43.
      Fernig, D., et al. 1994. Prog. Growth Factor Res. 5, 353.
      Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun. 157, 256.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2011 RFH
      SynonymsHBGF-2
      DescriptionRecombinant, rat fibroblast growth factor (FGF) basic consisting of amino acids 11-154 and expressed in E. coli. FGF basic, a member of the FGF family of mitogenic peptides, is known to bind heparine or cell surface heparin sulfate proteoglycans for binding, dimerization, and activation of tyrosine kinase FGF receptors. Also known to bind polysaccharides, proteins, and lipids with lower affinity.
      FormSolid
      FormulationLyophilized from 0.2 µm filtered MOPS, Na₂SO₄, EDTA, DTT, BSA.
      CAS number7757-82-6, 1132-61-2, 60-00-4, 9048-46-8, 106096-93-9, 3483-12-3
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Biological activityED₅₀ = 0.3-1.8 ng/ml as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.
      SolubilityReconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesPresta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
      Quarto, N., et al. 2005. Gene 356, 49.
      Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun. 335, 1239.
      Claus, P., et al. 2003. J. Biol. Chem. 278, 479.
      Coulier, F., et al. 1997. J. Mol. Evol. 44, 43.
      Fernig, D., et al. 1994. Prog. Growth Factor Res. 5, 353.
      Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun. 157, 256.