GF144 Epidermal Growth Factor Protein, Human recombinant

GF144
500 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      SourcePurity
      E.coli Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of EGF.
      Description
      Catalogue NumberGF144
      ReplacesGF001
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionEpidermal Growth Factor Protein, Human recombinant
      OverviewEpidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
      Alternate Names
      • EGF
      References
      Product Information
      PresentationLyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.
      Quality LevelMQ100
      Applications
      ApplicationThe Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.
      Application NotesFor most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.
      Biological Information
      PurityGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of EGF.
      SourceE.coli
      Specific ActivityHuman EGF is fully biologically active when compared to standards. The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.
      Entrez Gene Number
      Entrez Gene SummaryEpidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
      Gene Symbol
      • EGF
      • URG
      • urogastrone
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01133 # EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
      SIZE: 1207 amino acids; 133946 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P01133 ## Contains 9 EGF-like domains. & Contains 9 LDL-receptor class B repeats.
      Stem Cell Type
      • Neural Stem Cells
      • Epithelial Cells
      • Mesenchymal Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Epidermal Growth Factor Protein, Human recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      Epidermal Growth Factor Protein, Human recombinant Certificates of Analysis

      TitleLot Number
      EGF, HUMAN RECOMBINANT PROTEIN2845216
      EGF, HUMAN RECOMBINANT PROTEIN2934694
      EGF, HUMAN RECOMBINANT PROTEIN2919892
      EGF, HUMAN RECOMBINANT PROTEIN - 21357042135704
      EGF, HUMAN RECOMBINANT PROTEIN - 23847212384721
      EGF, HUMAN RECOMBINANT PROTEIN - 24441472444147
      EGF, HUMAN RECOMBINANT PROTEIN - 19887191988719
      EGF, HUMAN RECOMBINANT PROTEIN - 20524462052446
      EGF, HUMAN RECOMBINANT PROTEIN - 20660382066038
      EGF, HUMAN RECOMBINANT PROTEIN - 20737472073747

      Data Sheet

      Title
      EGF, HUMAN RECOMBINANT PROTEIN

      Newsletters / Publications

      Title
      Cellutions Newsletter: 2007, Volume 1
      Cellutions Newsletter: 2008, Volume 1
      Cellutions Newsletter: 2008, Volume 3

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors