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374096 Eosinophil Peroxidase, Human

374096
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374096-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewNative, human eosinophil peroxidase. Eosinophil peroxidase is a secreted hemoprotein that catalyzes oxidations of various substrates by hydrogen peroxide. Together with other leukocyte-derived enzymes, such as Myeloperoxidase (Cat. No. 475911), EPO plays an important role in the pathogenesis of asthma by mediating oxidative events that lead to the production of ROS (reactive oxygen species) and RNS (reactive nitrogen species).
      Catalogue Number374096
      Brand Family Calbiochem®
      SynonymsEPO
      References
      ReferencesAndreadis., et al. 2003. Free Radic. Biol. Med. 35, 213.
      Borelli, V., et al. 2003. Infect. Immun. 71, 605.
      Erpenbeck, V.J., et al. 2003. Clin. Exp. Allergy 33, 331.
      McElhinney, B., et al. 2003. J. Leukoc. Biol. 74, 897.
      Aldridge, R.E., et al. 2002. Free Radic. Biol. Med. 33, 847.
      Kettle, A.J., and Winterbourn, C.C. 1988. Biochim. Biophys. Acta 957, 185.
      Desser, R.K., et al. 1972. Arch. Biochem. Biophys. 148, 452.
      Hager, L.P., et al. 1966. J. Biol. Chem. 241, 1769.
      Product Information
      CAS number9003-99-0
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cause an increase in absorbance of 1 at 470 nm per min at pH 7.0 and 25°C, calculated from the initial linear rate of reaction using guaiacol as substrate.
      EC number1.11.1.7
      FormLyophilized
      FormulationLyophilized from 5 mM phosphate buffer, pH 7.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV and HIV.
      Specific Activity≥ 270 units/mg protein (Guaiacol Assay Method)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C). If solutions must be frozen the protein concentration should be >1 mg/ml and the activity determined following each thaw.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Eosinophil Peroxidase, Human SDS

      Title

      Safety Data Sheet (SDS) 

      Eosinophil Peroxidase, Human Certificates of Analysis

      TitleLot Number
      374096

      References

      Reference overview
      Andreadis., et al. 2003. Free Radic. Biol. Med. 35, 213.
      Borelli, V., et al. 2003. Infect. Immun. 71, 605.
      Erpenbeck, V.J., et al. 2003. Clin. Exp. Allergy 33, 331.
      McElhinney, B., et al. 2003. J. Leukoc. Biol. 74, 897.
      Aldridge, R.E., et al. 2002. Free Radic. Biol. Med. 33, 847.
      Kettle, A.J., and Winterbourn, C.C. 1988. Biochim. Biophys. Acta 957, 185.
      Desser, R.K., et al. 1972. Arch. Biochem. Biophys. 148, 452.
      Hager, L.P., et al. 1966. J. Biol. Chem. 241, 1769.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-July-2008 RFH
      SynonymsEPO
      DescriptionNative, human eosinophil peroxidase. Eosinophil peroxidase is a secreted hemoprotein that catalyzes the oxidation of various substrates by hydrogen peroxide. Together with other leukocyte-derived enzymes, such as Myeloperoxidase (Cat. No. 475911), EPO plays an important role in the pathogenesis of asthma by mediating oxidative events that lead to the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS).
      FormLyophilized
      FormulationLyophilized from 5 mM phosphate buffer, pH 7.0.
      SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV and HIV.
      CAS number9003-99-0
      EC number1.11.1.7
      Purity≥90% by SDS-PAGE
      Specific activity≥ 270 units/mg protein (Guaiacol Assay Method)
      Unit definitionOne unit is defined as the amount of enzyme that will cause an increase in absorbance of 1 at 470 nm per min at pH 7.0 and 25°C, calculated from the initial linear rate of reaction using guaiacol as substrate.
      SolubilityReconstitute in 400 µl sterile, distilled H₂O to yield a final concentration of ~1.7 µM.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C). If solutions must be frozen the protein concentration should be >1 mg/ml and the activity determined following each thaw.
      Toxicity Standard Handling
      ReferencesAndreadis., et al. 2003. Free Radic. Biol. Med. 35, 213.
      Borelli, V., et al. 2003. Infect. Immun. 71, 605.
      Erpenbeck, V.J., et al. 2003. Clin. Exp. Allergy 33, 331.
      McElhinney, B., et al. 2003. J. Leukoc. Biol. 74, 897.
      Aldridge, R.E., et al. 2002. Free Radic. Biol. Med. 33, 847.
      Kettle, A.J., and Winterbourn, C.C. 1988. Biochim. Biophys. Acta 957, 185.
      Desser, R.K., et al. 1972. Arch. Biochem. Biophys. 148, 452.
      Hager, L.P., et al. 1966. J. Biol. Chem. 241, 1769.