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324691 Elastatinal - CAS 51798-45-9 - Calbiochem

324691
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Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₂₁H₃₆N₈O₇ 51798-45-9

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324691-5MG
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      Plastic ampoule 5 mg
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      Description
      OverviewAn inhibitor of elastase-like serine protease produced by actinomycetes. Inhibits PMSF-stimulated degradation of histone H1 and HMG proteins. Also inhibits α2-macroglobulin-protease complexes. Inhibitory activity: ~0.5 µg/ml using Suc-(Ala)3-pNA as a substrate at 25°C, pH 8.0.
      Catalogue Number324691
      Brand Family Calbiochem®
      References
      ReferencesMenendez-Arias, L. 1992. J. Biol. Chem. 267, 11392.
      Umezawa, H. 1976. Methods Enzymol. 55, 678.
      Product Information
      CAS number51798-45-9
      ATP CompetitiveN
      FormYellow solid
      Hill FormulaC₂₁H₃₆N₈O₇
      Chemical formulaC₂₁H₃₆N₈O₇
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetElastase-like serine protease produced by actinomycetes
      Primary Target IC<sub>50</sub>~0.5 µg/ml using Suc-(Ala)3-pNA as a substrate at 25°C, pH 8.0.
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSLZ9600000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Elastatinal - CAS 51798-45-9 - Calbiochem Certificates of Analysis

      TitleLot Number
      324691

      References

      Reference overview
      Menendez-Arias, L. 1992. J. Biol. Chem. 267, 11392.
      Umezawa, H. 1976. Methods Enzymol. 55, 678.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      DescriptionElastase-like serine protease inhibitor produced by actinomycetes. Inhibits PMSF-stimulated degradation of histone H1 and HMG proteins. Also inhibits α2-macroglobulin-protease complexes. Effective at concentrations of 10-100 µM.
      FormYellow solid
      Recommended reaction conditions
      Assay Procedure 1. Dissolve enzyme in reaction buffer: 100 mM Tris-HCl, pH 7.5, 500 mM NaCl. 2. Prepare the substrate stock solution by dissolving Elastase Substrate I (MeOSuc-Ala-Ala-Pro-Val- pNA, Cat. No. 324696) at 50 mM in DMSO. 3. Just prior to assay, dilute the substrate stock solution with an equal volume of 200 mM Tris-HCl, pH 8.0. Mix well. 4. Prepare the assay mixture by combining 960 µl of reaction buffer with 40 µl of diluted substrate stock solution. 5. Add enzyme to assay mixture, mix, and incubate at 25°C. 6. Monitor the change in absorbance at 410 nm. Use an e of 8800 at 410 nm to calculate the hydrolysis of nitroanilide.
      CAS number51798-45-9
      RTECSLZ9600000
      Chemical formulaC₂₁H₃₆N₈O₇
      SolubilityH₂O (5 mg/ml)
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesMenendez-Arias, L. 1992. J. Biol. Chem. 267, 11392.
      Umezawa, H. 1976. Methods Enzymol. 55, 678.