324682 Elastase, Porcine Pancreas, High Purity, Crystallized

324682
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Overview

Replacement Information

Key Specifications Table

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324682-1000U
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      324682-250U
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          Description
          OverviewNative elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
          Catalogue Number324682
          Brand Family Calbiochem®
          References
          ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
          Mandl, I. 1962. Methods in Enzymol. 5, 665.
          Product Information
          CAS number39445-21-1
          Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=324699">Cat. No. 324699</a>) per min at 25°C, pH 8.0.
          EC number3.4.21.36
          FormLyophilized
          FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
          Quality LevelMQ100
          Applications
          Biological Information
          Specific Activity≥25 units/mg protein
          Physicochemical Information
          ContaminantsChymotrypsin: ≤0.2%
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSTS5052000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Elastase, Porcine Pancreas, High Purity, Crystallized SDS

          Title

          Safety Data Sheet (SDS) 

          Elastase, Porcine Pancreas, High Purity, Crystallized Certificates of Analysis

          TitleLot Number
          324682

          References

          Reference overview
          Largman, C. et al. 1976. Biochemestry 15, 2491.
          Mandl, I. 1962. Methods in Enzymol. 5, 665.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-July-2010 JSW
          DescriptionNative elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.
          FormLyophilized
          FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
          CAS number39445-21-1
          RTECSTS5052000
          EC number3.4.21.36
          ContaminantsChymotrypsin: ≤0.2%
          Specific activity≥25 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-pNA (Cat. No. 324699) per min at 25°C, pH 8.0.
          SolubilitydH₂O (10 mg/ml)
          Storage -20°C
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
          Toxicity Standard Handling
          Merck USA index14, 3535
          ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
          Mandl, I. 1962. Methods in Enzymol. 5, 665.

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          Categories

          Life Science Research > Proteins and Enzymes > Other Enzymes