Millipore Sigma Vibrant Logo

L2113-BC EDTA (Versen), 1 %

View Pricing & Availability


Replacement Information

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
Retrieving availability...
Fulfilment and delivery delayed
Fulfilment and delivery delayed
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      100 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
      Request Pricing
      OverviewTrypsin/EDTA solutions (0.05 or 0.02% in PBS, without Ca2+, Mg2+) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum.


      Functional Test: Pass
      Activity Test: Pass
      pH: 7.2-7.5
      Osmolality: 0.27-0.30 Osm/kg H20
      Color: Colorless
      Appearance: Clear

      Recommendation Instructions:

      Recommendations Trypsin (1:250)/EDTA solutions:
      Trypsin (1:250)/EDTA solutions (0.05/0.02 % in PBS without Ca2+, Mg2+) to passage monolayer cultures:
      1. Wash cell culture (monolayer) using physiological saline (w/o Ca2+, Mg2+).
      2. Apply a small quantity of Trypsin (1:250)/EDTA solution with a pipette to cell culture flask; a thin film is sufficient.
      3. Incubate culture flask at +37°C for approx. 5 min.
      4. Centrifuge cell suspension or transfer directly to a serum containing culture medium.

      With higher Trypsin concentrations, it is recommended to trypsinate at +2-+8°C.
      Catalogue NumberL2113-BC
      Brand Family Calbiochem®
      Product Information
      Quality LevelMQ300
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information