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371209 Dkk-2, Mouse, Recombinant

Dkk-2, Mouse, Recombinant, consists of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag® sequence.

Dkk-2, Mouse, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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371209
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Overview

Replacement Information

Key Specifications Table

Purity
≥95% by SDS-PAGE

Products

Catalog NumberPackaging Qty/Pack
371209-10UG Glass bottle 10 μg
Description
OverviewRecombinant, mouse Dickkopf related protein 2 (Dkk-2) consisting of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag® sequence. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations.
Catalogue Number371209
Brand Family Calbiochem®
References
ReferencesMorvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Mukhopadhyay, M. et al. 2006. Development 133, 2149.
Niehrs, C. 2006. Oncogene 25, 7469.
Li, X. et al. 2005. Nat. Genet. 37, 945.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. and C. Niehrs 2003. Gene 302, 179.
Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol. 22, 6100.
Li, L. et al. 2002. J. Biol. Chem. 277, 5977.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Wu, W. et al. 2000. Current Biol. 10,1611.
Monaghan, A.P. et al. 1999. Mech. Dev. 87, 45.
Krupnik, V.E. et al. 1999. Gene 238, 301.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityInhibition assay:
Inhibits WNT-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells in the presence of BMP-2.

Competetive Binding Assay:
Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU per/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
371209-10UG 04055977213836

Documentation

Dkk-2, Mouse, Recombinant SDS

Title

Safety Data Sheet (SDS) 

Dkk-2, Mouse, Recombinant Certificates of Analysis

TitleLot Number
371209

References

Reference overview
Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Mukhopadhyay, M. et al. 2006. Development 133, 2149.
Niehrs, C. 2006. Oncogene 25, 7469.
Li, X. et al. 2005. Nat. Genet. 37, 945.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. and C. Niehrs 2003. Gene 302, 179.
Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol. 22, 6100.
Li, L. et al. 2002. J. Biol. Chem. 277, 5977.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Wu, W. et al. 2000. Current Biol. 10,1611.
Monaghan, A.P. et al. 1999. Mech. Dev. 87, 45.
Krupnik, V.E. et al. 1999. Gene 238, 301.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-September-2011 RFH
DescriptionRecombinant, mouse Dickkopf related protein 2 (Dkk-2) consisting of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag® sequence. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations.
FormSolid
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU per/µg protein
Biological activityInhibition assay:
Inhibits WNT-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells in the presence of BMP-2.

Competetive Binding Assay:
Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
SolubilityReconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesMorvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
Mukhopadhyay, M. et al. 2006. Development 133, 2149.
Niehrs, C. 2006. Oncogene 25, 7469.
Li, X. et al. 2005. Nat. Genet. 37, 945.
Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
Mao, B. and C. Niehrs 2003. Gene 302, 179.
Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol. 22, 6100.
Li, L. et al. 2002. J. Biol. Chem. 277, 5977.
Mao, B. et al. 2002. Nature 417, 664.
Mao, B. et al. 2001. Nature 411, 321.
Wu, W. et al. 2000. Current Biol. 10,1611.
Monaghan, A.P. et al. 1999. Mech. Dev. 87, 45.
Krupnik, V.E. et al. 1999. Gene 238, 301.