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662141 DUB Inhibitor V, PR-619 - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₇H₅N₅S₂

Products

Catalog NumberPackaging Qty/Pack
662141-25MG Glass bottle 25 mg
Description
OverviewA cell-permeable pyridinamine class broad-spectrum DUB inhibitor whose known targets include ATXN3, BAP1, JOSD2, OTUD5, UCH-L1, UCH-L3, UCH-L5/UCH37, USP1, 2, 4, 5, 7, 8, 9X, 10, 14, 15, 16, 19, 20, 22, 24, 28, 47, 48, VCIP135, YOD1, as well as deISGylase PLpro, deNEDDylase DEN1, and deSUMOlyase SENP6. Both PR-619 and P22077 (Cat. No. 662142) are shown to increase overall protein polyubiquitination in HEK293T cells in a dose- and time-dependent manner (20 to 150 µM; 0.5 to 20 h), however P22077 exposure results in mainly enrichment of K48-linked, while PR619 treatment results in upregulation of both K48- and K63-linked polyUb chains.
Catalogue Number662141
Brand Family Calbiochem®
SynonymsPR619, UCH-L1 Inhibitor IV, UCH-L3 Inhibitor II, UCH-L5/UCH37 Inhibitor III, USP5 Inhibitor II, USP7 Inhibitor I, USP9X Inhibitor II, USP14 Inhibitor IV, USP14 Inhibitor IV, USP47 Inhibitor I, 2,6-Diaminopyridine-3,5-bis(thiocyanate), 3,5-Dithiocyanatopyridine-2,6-diamine
References
ReferencesAltun, M., et al. 2011. Chem. Biol. 18, 1401.
Tian, X., et al. 2011. Assay Drug Dev. Technol. 9, 165.
Product Information
FormBeige solid
Hill FormulaC₇H₅N₅S₂
Chemical formulaC₇H₅N₅S₂
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationDUB Inhibitor V, PR-619, CAS 21645-32-1, is a cell-permeable, broad-spectrum DUB inhibitor whose (C50 = 5-20 M).
Biological Information
Purity≥99% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
662141-25MG 04055977261240

Documentation

DUB Inhibitor V, PR-619 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

DUB Inhibitor V, PR-619 - Calbiochem Certificates of Analysis

TitleLot Number
662141

References

Reference overview
Altun, M., et al. 2011. Chem. Biol. 18, 1401.
Tian, X., et al. 2011. Assay Drug Dev. Technol. 9, 165.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-November-2012 JSW
SynonymsPR619, UCH-L1 Inhibitor IV, UCH-L3 Inhibitor II, UCH-L5/UCH37 Inhibitor III, USP5 Inhibitor II, USP7 Inhibitor I, USP9X Inhibitor II, USP14 Inhibitor IV, USP14 Inhibitor IV, USP47 Inhibitor I, 2,6-Diaminopyridine-3,5-bis(thiocyanate), 3,5-Dithiocyanatopyridine-2,6-diamine
DescriptionA cell-permeable pyridinamine class broad-spectrum DUB inhibitor whose known targets include ATXN3, BAP1, JOSD2, OTUD5, UCH-L1, UCH-L3, UCH-L5/UCH37, USP1, 2, 4, 5, 7, 8, 9X, 10, 14, 15, 16, 19, 20, 22, 24, 28, 47, 48, VCIP135, YOD1, as well as deISGylase PLpro, deNEDDylase DEN1, and deSUMOlyase SENP6. Both PR-619 and P22077 (Cat. No. 662142) are shown to increase overall protein polyubiquitination in HEK293T cells in a dose- and time-dependent manner (20 to 150 µM; 0.5 to 20 h), however P22077 exposure results in mainly enrichment of K48-linked, while PR619 treatment results in upregulation of both K48- and K63-linked polyUb chains.
FormBeige solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₇H₅N₅S₂
Structure formulaStructure formula
Purity≥99% by HPLC
SolubilityDMSO (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesAltun, M., et al. 2011. Chem. Biol. 18, 1401.
Tian, X., et al. 2011. Assay Drug Dev. Technol. 9, 165.