Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on More

69725 λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen

View Products on
Purchase on Sigma-Aldrich


Replacement Information


Catalog NumberPackaging Qty/Pack
69725-3 Glass bottle 10 rxn
OverviewThe λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69725
Brand Family Novagen®
Product Information
200 µlλDE3 Phage Lysate
200 µlHelper Phage Lysate
200 µlSelection Phage Lysate
50 µlT7 Tester Phage Lysate
200 µlHMS174(DE3) glycerol stock
1 µgpLysS DNA
1 µgpLysE DNA
Quality LevelMQ100
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
69725-3 04055977258981


λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen SDS


Safety Data Sheet (SDS) 

λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen Certificates of Analysis

TitleLot Number


  • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
  • Junhua Zhao and Alan M. Lambowitz. (2005) A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles. Proceedings of the National Academy of Sciences (USA) 102, 16133-16140.
  • Dhammika Gunasekera and Robert G. Kemp. (1999) Cloning, sequencing, expression, and purification of the C isozyme of mouse phosphofructokinase. Protein Expression and Purification 16, 448-453.
  • User Protocols

    TB053 Academic and Non-profit Laboratory Assurance Letter

    Vector Map

    TB107 pLysS and pLysE Plasmid Map