71009 CytoBuster™ Protein Extraction Reagent

71009
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Overview

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71009-3
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      Plastic ampoule 50 ml
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      71009-4
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          Glass bottle 250 ml
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          Description
          OverviewThe CytoBuster™ Protein Extraction Reagent is a proprietary formulation of detergents optimized for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of CytoBuster Protein Extraction Reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw. CytoBuster Protein Extraction Reagent has been specifically formulated for utilization in Western blotting, immunoprecipitation, and kinase/phosphatase assays. The reagent is compatible with protease inhibitors, kinase inhibitors, and phosphatase inhibitors.


          Catalogue Number71009
          Brand Family Novagen®
          References
          Product Information
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice
          Toxicity Standard Handling
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          CytoBuster™ Protein Extraction Reagent SDS

          Title

          Safety Data Sheet (SDS) 

          CytoBuster™ Protein Extraction Reagent Certificates of Analysis

          TitleLot Number
          71009

          Brochure

          Title
          Protein Purification and Detection Tools
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (MilliporeSigma)

          Technical Info

          Title
          Western Blotting Quick Reference Product Guide

          Citations

          Title
        • Kar Wah Leung, et al. (2008) Expression of ZnT and ZIP zinc transporters in the human RPE and their regulation by neurotrophic factors. Investigative Opthalmology and Visual Science 49, 1221-1231.
        • Chen-Hong Li, et al. (2008) Src tyrosine kinase alters gating of hyperpolarization-activated HCN4 pacemaker channel through Tyr531. American Journal of Physiology- Cell Physiology 294, C355-C362.
        • Yuk Cheung Chan and Po Sing Leung. (2007) Angiotensin II type 1 receptor-dependent nuclear factor-κB activation-mediated proinflammatory actions in a rat model of obstructive acute pancreatitis. Journal of Pharmacology and Experimental Therapeutics 323, 10-18.
        • Xiao-Yu Li, et al. (2007) APOBEC3G inhibits DNA strand transfer during HIV-1 reverse transcription. Journal of Biological Chemistry 282, 32065-32074.
        • Keyang Chen, et al. (2006) Alpha interferon potently enhances the anti-human immunodeficiency virus type 1 activity of APOBEC3G in resting primary CD4 T cells. Journal of Virology 80, 7645-7657.
        • Sun-Young Chang, et al. (2005) Heparan sulfate regulates the antiangiogenic activity of EMAP II at acidic pH. Molecular Pharmacology 67, 1534-1543.
        • Laurent Plantier, et al. (2005) Defect of hepatocyte growth factor production by fibroblasts in human pulmonary emphysema. American Journal of Physiology: Lung 288, L641-L647.
        • T. Michael Redmond, et al. (2005) Mutation of key residues of RPE65 abolishes its enzymatic role as isomerohydrolase in the visual cycle. Proceedings of the National Academy of Sciences (USA) 102, 13658-13663.
        • Dongqing Wang and S. Stoney Simons, Jr.. (2005) Corepressor binding to progesterone and glucocorticoid receptors involves the activation function-1 domain and is inhibited by molybdate. Molecular Endocrinology 19, 1483-1500.
        • Franziska Boess, et al. (2003) Gene expression in two hepatic cell lines, cultured primary hepatocytes and liver slices compared to the in vivo liver gene expression in rats: possible implications for toxicogenomics use of in vitro systems. Toxicological Science 73, 386-402.
        • Anne F. McGettrick, Ruth C. Barnes and D. Margaret Worrall. (2001) SCCA2 inhibits TNF-mediated apoptosis in transfected HeLa cells. European Journal of Biochemistry 268, 5868-5875.
        • User Protocols

          Title
          TB306 CytoBuster™ Protein Extraction Reagent