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L7213-BC Collagen G

L7213-BC
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
L7213 100 ml
Description
OverviewGrowth and differentiation of cells in vitro are significantly influenced by the substrate used (glass or plastic). If culture flasks are coated with attachment factors such as Fibronectin, Collagen, Gelatine or Polylysine, better cell growth is often achieved. Collagen G is used in the production of gels, to embed cells. Collagen G gels are suitable for use as a substrate for adherent cells in a cell culture vessel and as a floating matrix in or on cell culture medium. 0.4% solution in 15 mmol/l HCl, type I, 4 mg/ml

Specifications:

Cell Attachment: Pass
pH: 1.8-2.4
Osmolality: 0.01-0.05 Osm/kg H20
Collagen Content: 0.37-0.45%
Hydroxyproline: 0.046-0.0565%
Color: Colorless
Appearance: Clear

Collagen G for optimum geling
We sell two different collagens: Collagen A (cat. no. L7220) and Collagen G (cat. no. L7213), acid-soluble calfskin collagens. Collagen G is used in the production of gels, to embed cells. Collagen G gels are suitable for use as a substrate for adherent cells in a cell culture vessel and as a floating matrix in or on cell culture medium. (Collagen A is used to coat culture flasks.) We have updated the recommendations for the manufacture of gels with Collagen G with regard to the concentration and the necessary pH values of the starting solutions, to optimise geling. New features include tips for examining gels under a microscope and taking pictures.

Components for gel production
Other components, also offered by us, are required for the production of Collagen G gels (cat. no. L7213). You can find an overview of these in Table 48.
Tab. 48: Components required for gel production

Recommendations for optimum geling with Collagen G

Geling is heavily influenced by the pH value. Before starting work, pre-cool all reagents to a temperature of +2 - +8°C.

Solution A:
0.7 M sodium hydroxide and 1 M HEPES buffer (cat. no. L 1613) are mixed equally (e.g. 5 ml sodium hydroxide with 5 ml HEPES buffer).
Solution B:
A 10x medium and solution A are mixed equally (e.g. 5 ml medium with 5 ml solution A).
• The pH of solution B should be between 7.90 and 8.05. It is advisable to check the pH using a suitable measuring method. If the gel should remain sterile, measure the pH of an aliquot previously removed.
• 8.0 ml Collagen G is gently mixed with 2.0 ml of solution B to produce the ready-to-use solution for geling. This prevents the formation of air bubbles.
• 3.0 ml of solution prepared in this way is pipetted into a 25 cm2 culture flask. For a culture flask with a diameter of 9 cm, 5.0 ml is required. The culture flask is then incubated vibration-free for at least 1 hour or, for optimum stability, 24 hours at +35°C (± 2°C).

Examining the gel produced under a microscope and taking pictures
If a 35 mm culture dish is filled with 2 ml Collagen G/buffer media mixture (pH approx. 7.2 - 7.8) and incubated, a gel with faint white diffusion is formed, viewed from above. This diffusion appears as a result of crosslinking of the collagen molecules and should not cause any problems when examining under a microscope or taking pictures.
The accumulation of diffusion is proportional to the increase of thickness of the gel, for example if 5-7 ml instead of 2 ml per 10 cm2 Collagen G (35 mm culture vessel) is used. This could make microscopic and photographic analysis difficult.
Catalogue NumberL7213-BC
Brand Family Calbiochem®
References
Product Information
Quality LevelMQ300
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Storage +2°C to +25°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
L7213 04027269841386

Documentation