227041 Sigma-AldrichCholera Toxin, B Subunit, Type Inaba 569B, Peroxidase Conjugate - Calbiochem

Suitable for the demonstration of dendritic branching in retrogradely labeled neurons.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Recommended Products

Overview

Replacement Information

Key Specifications Table

Pricing & Availability

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Description
Overview	Suitable for the demonstration of dendritic branching in retrogradely labeled neurons. Shown to be up to 50 times more sensitive than free peroxidase as both an orthogradely and retrogradely transported marker in the rat visual system.
Overview	Sold on the basis of HRP content.
Catalogue Number	227041
Brand Family	Calbiochem®

References
References	Raappana, P., and Arvidsson, J. 1993. J. Comp. Neurol. 328, 103. Trojanowski, J.Q. 1983. J. Neurosci. Methods 9, 185. Trojanowski, J.Q., et al. 1982. Brain Res. 231, 33. Wan, X.S.T., et al. 1982. Exp. Neurol. 78, 167. Avrameas, S., and Ternynck, T. 1971. Immunochemistry 8, 1175.

Product Information
ATP Competitive	N
Form	Solid
Formulation	Lyophilized from 10 mM sodium phosphate buffer, pH 7.5.
Reversible	N
Quality Level	MQ100

Applications

Biological Information
Primary Target	Demonstration of dendritic branching in retrogradely labeled neurons
Source	Prepared by a modification of the two-step conjugation method of Avrameas, S., and Ternynck, T. 1971. Chromatographically purified and lyophilized.
Specific Activity	≥125 purpurogallin units/mg HRP

Physicochemical Information
Cell permeable	N

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 20/21/22-36/37/38-52/53 Harmful by inhalation, in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin. Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
S Phrase	S: 26-36-61 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. Avoid release to the environment. Refer to special instructions/safety data sheet.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Harmful
Storage	+2°C to +8°C
Do not freeze	Yes
Special Instructions	Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
End use certificate	Y
Canadian export regulations	Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.

Packaging Information

Transport Information

Supplemental Information

Specifications

Documentation

Cholera Toxin, B Subunit, Type Inaba 569B, Peroxidase Conjugate - Calbiochem SDS

Title
Safety Data Sheet (SDS)

Cholera Toxin, B Subunit, Type Inaba 569B, Peroxidase Conjugate - Calbiochem Certificates of Analysis

Title	Lot Number
227041	Enter Lot Number

References

Reference overview
Raappana, P., and Arvidsson, J. 1993. J. Comp. Neurol. 328, 103. Trojanowski, J.Q. 1983. J. Neurosci. Methods 9, 185. Trojanowski, J.Q., et al. 1982. Brain Res. 231, 33. Wan, X.S.T., et al. 1982. Exp. Neurol. 78, 167. Avrameas, S., and Ternynck, T. 1971. Immunochemistry 8, 1175.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	05-June-2008 RFH
Description	Suitable for the demonstration of dendritic branching in retrogradely labeled neurons. Has been shown to be up to 50 times more sensitive than free peroxidase as both an orthogradely and retrogradely transported marker in the rat visual system.
Form	Solid
Formulation	Lyophilized from 10 mM sodium phosphate buffer, pH 7.5.
Source	Prepared by a modification of the two-step conjugation method of Avrameas, S., and Ternynck, T. 1971. Chromatographically purified and lyophilized.
Specific activity	≥125 purpurogallin units/mg HRP
Solubility	Reconstitute in ≤100 µl of sterile distilled H₂O.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Special Instructions	Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Toxicity	Harmful
References	Raappana, P., and Arvidsson, J. 1993. J. Comp. Neurol. 328, 103. Trojanowski, J.Q. 1983. J. Neurosci. Methods 9, 185. Trojanowski, J.Q., et al. 1982. Brain Res. 231, 33. Wan, X.S.T., et al. 1982. Exp. Neurol. 78, 167. Avrameas, S., and Ternynck, T. 1971. Immunochemistry 8, 1175.