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227041 Cholera Toxin, B Subunit, Type Inaba 569B, Peroxidase Conjugate - Calbiochem

Suitable for the demonstration of dendritic branching in retrogradely labeled neurons.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information
Description
OverviewSuitable for the demonstration of dendritic branching in retrogradely labeled neurons. Shown to be up to 50 times more sensitive than free peroxidase as both an orthogradely and retrogradely transported marker in the rat visual system.
Sold on the basis of HRP content.
Catalogue Number227041
Brand Family Calbiochem®
References
ReferencesRaappana, P., and Arvidsson, J. 1993. J. Comp. Neurol. 328, 103.
Trojanowski, J.Q. 1983. J. Neurosci. Methods 9, 185.
Trojanowski, J.Q., et al. 1982. Brain Res. 231, 33.
Wan, X.S.T., et al. 1982. Exp. Neurol. 78, 167.
Avrameas, S., and Ternynck, T. 1971. Immunochemistry 8, 1175.
Product Information
ATP CompetitiveN
FormSolid
FormulationLyophilized from 10 mM sodium phosphate buffer, pH 7.5.
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetDemonstration of dendritic branching in retrogradely labeled neurons
SourcePrepared by a modification of the two-step conjugation method of Avrameas, S., and Ternynck, T. 1971. Chromatographically purified and lyophilized.
Specific Activity≥125 purpurogallin units/mg HRP
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/21/22-36/37/38-52/53

Harmful by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
S PhraseS: 26-36-61

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Avoid release to the environment. Refer to special instructions/safety data sheet.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
End use certificateY
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Identification Number
Catalog Number GTIN
227041

Documentation

Cholera Toxin, B Subunit, Type Inaba 569B, Peroxidase Conjugate - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Cholera Toxin, B Subunit, Type Inaba 569B, Peroxidase Conjugate - Calbiochem Certificates of Analysis

TitleLot Number
227041

References

Reference overview
Raappana, P., and Arvidsson, J. 1993. J. Comp. Neurol. 328, 103.
Trojanowski, J.Q. 1983. J. Neurosci. Methods 9, 185.
Trojanowski, J.Q., et al. 1982. Brain Res. 231, 33.
Wan, X.S.T., et al. 1982. Exp. Neurol. 78, 167.
Avrameas, S., and Ternynck, T. 1971. Immunochemistry 8, 1175.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-June-2008 RFH
DescriptionSuitable for the demonstration of dendritic branching in retrogradely labeled neurons. Has been shown to be up to 50 times more sensitive than free peroxidase as both an orthogradely and retrogradely transported marker in the rat visual system.
FormSolid
FormulationLyophilized from 10 mM sodium phosphate buffer, pH 7.5.
SourcePrepared by a modification of the two-step conjugation method of Avrameas, S., and Ternynck, T. 1971. Chromatographically purified and lyophilized.
Specific activity≥125 purpurogallin units/mg HRP
SolubilityReconstitute in ≤100 µl of sterile distilled H₂O.
Storage +2°C to +8°C
Do Not Freeze Yes
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Toxicity Harmful
ReferencesRaappana, P., and Arvidsson, J. 1993. J. Comp. Neurol. 328, 103.
Trojanowski, J.Q. 1983. J. Neurosci. Methods 9, 185.
Trojanowski, J.Q., et al. 1982. Brain Res. 231, 33.
Wan, X.S.T., et al. 1982. Exp. Neurol. 78, 167.
Avrameas, S., and Ternynck, T. 1971. Immunochemistry 8, 1175.