17-608 ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set

17-608
10 assays  10 assays per kit, ~4μL per chromatin immunoprecipitation
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, M WB, ChIP
      Description
      Catalogue Number17-608
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ HDAC1 set includes the HDAC1 antibody, the negative control antibody supernatant (mouse IgG), and qPCR primers flanking an Sp1 binding site in human p21 (WAF1/CIP1/CDKN1A) promoter, amplifying a 105 base pair PCR product. The HDAC1 and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC1 associated chromatin.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Included negative control antibody supernatant mouse IgG and control primers specific for human p21 (WAF1/CIP1/CDKN1A) promoter.
      PresentationAnti-HDAC1 (mouse monoclonal IgG1,supernatant). One vial containing 40 μL of culture supernatant with 0.05% sodium azide. Store at -20°C.

      Negative ChIP Control Supernatant. One vial containing 40 uL of mouse IgG containing supernatant with 0.05% sodium azide. Store at -20°C.

      ChIP Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at -20°C.
      P21 Forward: CCC ACA GCA GAG GAG AAA GAA
      P21 Reverse: CTG GAA ATC TCT GCC CAG ACA
      Quality LevelMQ100
      Applications
      ApplicationThis ChIPAb+ HDAC1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation Analysis:
      Sonicated chromatin prepared from untreated or actinomycin D treated (30 ng/mL, 6 hrs.) U2OS cells (3 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 4 μL of Mouse Anti-HDAC1 and the Magna ChIP™ G (Cat. # 17-611) Kit. Immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers p21 flanking the human p21 promoter that contains an Sp1 binding site (Please see figures).
      Fold Decrease is a ratio of normalized mean IP quantities extracted from standard curves derived from inputs of each chromatin sample. As previously published, HDAC1 association with this region of the p21 promoter decreases upon actinomycin D treatment.

      Western Blot Analysis:
      3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC1 (1:2500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
      Biological Information
      ImmunogenThe HDAC1 antibody is made against a peptide corresponding to amino acids 467-482 of mouse HDAC1.
      HostMouse
      SpecificityHDAC1
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HD1
      • HDAC1
      • RPD3L1
      UniProt Number
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by chromatin immunoprecipitation on U2OS cells and immunoblot on 3T3/A31 nuclear lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt
      Packaging Information
      Material Size10 assays
      Material Package10 assays per kit, ~4μL per chromatin immunoprecipitation
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set SDS

      Title

      Safety Data Sheet (SDS) 

      ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set Certificates of Analysis

      TitleLot Number
      ChIPAb+ HDAC1 - 24204642420464
      ChIPAb+ HDAC1 - 19593101959310
      ChIPAb+ HDAC1 - 20160242016024
      ChIPAb+ HDAC1 - 20891412089141
      ChIPAb+ HDAC1 - 21941502194150
      ChIPAb+ HDAC1 - 33860333386033
      ChIPAb+ HDAC1 - DAM1663272DAM1663272
      ChIPAb+ HDAC1 - DAM1732843DAM1732843
      ChIPAb+ HDAC1 - DAM1754489DAM1754489
      ChIPAb+ HDAC1 - DAM1764572DAM1764572

      References

      Reference overviewPub Med ID
      Knockdown of menin affects pre-mRNA processing and promoter fidelity at the interferon-gamma inducible IRF1 gene.
      Auriemma, LB; Shah, S; Linden, LM; Henriksen, MA
      Epigenetics & chromatin  5  2  2012

      Show Abstract
      22240255 22240255
      cAMP-responsive element modulator (CREM)α protein signaling mediates epigenetic remodeling of the human interleukin-2 gene: implications in systemic lupus erythematosus.
      Hedrich, Christian M, et al.
      J. Biol. Chem., 286: 43429-36 (2011)  2011

      Show Abstract
      21976679 21976679
      cAMP-responsive element modulator (CREM)α protein induces interleukin 17A expression and mediates epigenetic alterations at the interleukin-17A gene locus in patients with systemic lupus erythematosus.
      Rauen, Thomas, et al.
      J. Biol. Chem., 286: 43437-46 (2011)  2011

      Show Abstract
      22025620 22025620
      A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells.
      Cantu', C; Grande, V; Alborelli, I; Cassinelli, L; Cantu', I; Colzani, MT; Ierardi, R; Ronzoni, L; Cappellini, MD; Ferrari, G; Ottolenghi, S; Ronchi, A
      Nucleic acids research  39  486-501  2011

      Show Abstract
      20852263 20852263
      Repression of E-cadherin by the polycomb group protein EZH2 in cancer.
      Cao, Q; Yu, J; Dhanasekaran, SM; Kim, JH; Mani, RS; Tomlins, SA; Mehra, R; Laxman, B; Cao, X; Yu, J; Kleer, CG; Varambally, S; Chinnaiyan, AM
      Oncogene  27  7274-84  2008

      Show Abstract
      18806826 18806826

      Brochure

      Title
      Advance your Epigenetics Research (MilliporeSigma)

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      Product Families

      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies