17-10135 ChIPAb+ CBX8 - ChIP Validated Antibody and Primer Set

17-10135
25 assays  25 assays per set. Recommended use: 2.0 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H ChIP, WB
      Description
      Catalogue Number17-10135
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ CBX8 - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ CBX8 set includes a CBX8 antibody, a Normal Rabbit IgG, and positive control primers which amplify 138 bp of the human HOXA2 promoter region. The CBX8 antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of CBX8 associated chromatin.
      Alternate Names
      • Polycomb 3 homolog
      • Rectachrome 1
      • Polycomb 3 homolog
      • hPc3
      • Pc3
      Background InformationThe family of Heterochromatin Proteins ("Chromobox Homolog", CBX) are highly conserved adapter molecules, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.
      References
      Product Information
      FormatPurified
      Control
      • Includes normal rabbit IgG and primers specific for the human HOXA2 promoter region.
      PresentationAnti-CBX8 (Rabbit Polyclonal), Part No. CS207348. One vial containing 50 µg affinity purified rabbit polyclonal in 72 uL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7 mg/ml final). Store at -20°C.

      Normal Rabbit IgG, Part No. PP64B. One vial containing 125 μg of purified Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C.

      ChIP Primers, HOXA2 Upstream. Part No. CS203200. One vial containing 75 μL of 5 μM of each primer specific for human HOXA2 promoter region (chr7:27142948-27143085, hg19 build). Store at -20°C.
      FOR: AGG AAA GAT TTT GGT TGG GAA G
      REV: AAA AAG AGG GAA AGG GAC AGA C
      Quality LevelMQ100
      Applications
      ApplicationUse ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 & Polycomb 3 homolog.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      Application NotesChromatin Immunoprecipitation:
      Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-CBX8 (Part No. CS207348) and the Magna ChIP™ HiSens (Cat. # 17-10460). Successful immunoprecipitation of CBX8 associated DNA fragments was verified by qPCR using ChIP Primers, HoxA2 upstream (Part No. CS203200) as a positive locus, and GAPDH promoter primers (Part No. 22-004) as a negative locus. Data are presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
      Please refer to the Magna ChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens(Cat. # 17-10461) protocol for experimental details.

      Chromatin Immunoprecipitation:
      Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min ( 1e5 and 1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-CBX8 (Part No. CS207348) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of CBX8 associated DNA fragments was verified by qPCR using ChIP Primers, Hox A2 upstream (Part No. CS203200).
      Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected CBX8 on 5, 15 and 50 µg of HeLa whole cell lysate and 50 µg of 293T whole cell lysate.
      Biological Information
      EpitopeHuman CBX8 (a.a. 350--389)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Wide species reactivity expected based upon conservation of immunogen sequence
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HPC3
      • PC3
      • Pc3
      • RC1
      • hPc3
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~43kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-CBX8 (Part No. CS207348) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of CBX8 associated DNA fragments was verified by qPCR using ChIP Primers, HoxA2 upstream (Part No. CS203200).
      Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. Recommended use: 2.0 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies