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Centrifree® Ultrafiltration Device

Separate free from bound solutes

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Overview

Specifications

Ordering Information

Centrifree Ultrafiltration DeviceClear Sorting & Filtering Show Filter
Catalog NumberDescriptionPack Size
4104Centrifree Ultrafiltration Device with Ultracel PL membrane 50 Show Pricing & Availability

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Documentation

References

Reference overviewApplication
Fractionation of canine serum magnesium.
Schenck PA.,Vet Clin Pathol. 2005 Jun;34(2):137-9.
Vet Clin Pathol. 2005 Jun;34(2):137-9.  2005

The indirect effect of Tityus discrepans on rabbit pulmonary vasculature.
Novoa, Eva, et al.
Respiratory physiology & neurobiology, 134: 33-41 (2003)  2003

Show Abstract
Cell Culture
Metabolic activity of cultured rat brainstem, hippocampal and spinal cord slices.
Caroline A. Connelly, Lawrence C. Chen and Steven D. Colquhoun
Journal of Neuroscience Methods 99 (1-2): 1-7  2000

Cell Culture
Adaptation of total phenytoin reagent pack for measuring free phenytoin levels with the Abbott AxSYM immunoassay analyzer
Jarzabek JI (REPRINT) ; Kampa IS
Therapeutic drug monitoring 1999, v21, n1, p134-136  1999

Phenylalanine and tyrosine quantification by stable isotope dilution liquid chromatography-mass spectrometry from filter paper blood spots
Tuchman, M. McCann, M. T.
Clinical Chemistry (Washington, D. C.) VO- 45 IS- 4 PG- 571-573 Apr 1999  1999

Sample Prep
Circadian rhythm of free melatonin in human plasma
Kennaway DJ (REPRINT) ; Voultsios A
JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM, 1998, V83, N3 MAR, P1013-1015  1998

Clinical Lab, Protein Binding
Factors affecting the free plasma fraction of phenytoin in patients with epilepsy
Jimenez MC; Duran JA (REPRINT) ; Abadin JA
Clinical drug investigation, 1998, V15, P153-157  1998

Clinical Lab
Differential analysis of blood urea using combined automated ultrafiltration and solid phase extraction in on-line series
Shintani H.,Journal of Liquid Chromatography and Related Technologies (2205-2210) 1998
Journal of Liquid Chromatography and Related Technologies (2205-2210) 1998  1998

Effects of tryptophan depletion vs. catecholamine depletion in patients with seasonal affective disorder in remission with light therapy
Neumeister, Alexander et al.
Archives of general psychiatry, 1998  1998

Valproic acid-ketoconazole interaction in normal, hypoalbuminemic, and uremic sera: Lack of interaction in uremic serum caused by the presence of inhibitor
Dasgupta Amitava(a); Luke Matthew; Therapeutic Drug Monitoring VO- 19 IS- 3 PG- 281-285 June, 1997 SN- 0163-4356
Therapeutic Drug Monitoring VO- 19 IS- 3 PG- 281-285 June, 1997 SN- 0163-4356  1997

FAQ

QuestionAnswer
Can I use Centrifree with a swinging-bucket rotor?Yes, but the filtration rate will be only 30-50% of that produced with a fixed-angle rotor at the same g-force.
What is the maximum spinning speed of the Centrifree unit?Device limits are measured in g-force, not rpm. The maximum recommended g-force is 2000 × g.
How much ultrafiltrate can I expect when using the Centrifree?For volumes less than 0.5 ml: For a sample volume of 1.0 ml: In 10 minutes: I 1/2 the initial volume approximately 200 µl In 30 minutes: 2/3 to 3/4 the initial volume approximately 450 µl
What is the maximum process volume for the Centrifree device?Maximum volume is 1.0 ml.
What molecular weight cut-off does the Centrifree come in?The Centrifree unit is available in a 30,000 MW cut-off.
Can I reuse Centrifree?No, Centrifree is intended for one-time use only. Multiple use risks membrane failure.
What is the minimum process volume for the Centrifree device?Minimum volume is 0.15 ml.
What temperatures can Centrifree be spun at?Spin devices at 4-50°C. Store at room temperature.
What centrifuge can I use with the Centrifree?Any centrifuge with a fixed angle rotor or carrier that can accommodate 17 × 100 mm tubes and is capable of 1000-2000 g.
How long do I spin the Centrifree?1ml of normal human serum spun in a fixed angle rotor will produce 125-130ul of ultrafiltrate in 10 minutes. 1ml of normal human serum spun in a swinging bucket will produce 115-120ul of ultrafiltrate in 10 minutes.