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70794 BugBuster® GST•Bind™ Purification Kit

70794
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      Description
      Overview

      The BugBuster® GST•Bind™ Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag™ fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.



      Catalogue Number70794
      Brand Family Novagen®
      References
      Product Information
      2 x 100 mlBugBuster Protein Extraction Reagent
      10,000 UBenzonase Nuclease, purity > 90%
      10 mlGST•Bind Resin
      pkg/4Chromatography Columns
      2 x 100 ml10X GST Bind/Wash Buffer
      40 ml10X Glutathione Reconstitution Buffer
      1 gGlutathione, Reduced
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/38

      Irritating to eyes and skin.
      S PhraseS: 26

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      BugBuster® GST•Bind™ Purification Kit SDS

      Title

      Safety Data Sheet (SDS) 

      BugBuster® GST•Bind™ Purification Kit Certificates of Analysis

      TitleLot Number
      70794

      Brochure

      Title
      Protein Purification and Detection Tools

      Citations

      Title
    • Xiaobing Chen, et al. (2008) Organization of the core structure of the postsynaptic density. Procedings of the National Academy of Science 105, 4453-4458.
    • Bryan T. Carroll, et al. (2006) Sulfated signal from ASJ sensory neurons modulates stomatin-dependent coordination in Caenorhabditis elegans. Journal of Biological Chemistry 281, 35989-35996.
    • Laigeng Li, et al. (2005) Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen. Plant and Cell Physiology 46, 1073-1082.
    • User Protocols

      Title
      TB235 GST•Bind™ Kits
      TB245 BugBuster® Protein Extraction Reagent