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203291 Bisindolylmaleimide I, Hydrochloride - CAS 176504-36-2 - Calbiochem

Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
176504-36-2C₂₅H₂₄N₄O₂ · HCl

Products

Catalog NumberPackaging Qty/Pack
203291-1MG Plastic ampoule 1 mg
203291-250UG Plastic ampoule 250 μg
Description
OverviewAn enhanced water-soluble form of Bisindolylmaleimide I (Cat. No. 203290). A reversible and ATP-competitive inhibitor of protein kinase C (Ki = 10 nM). May inhibit protein kinase A at much higher concentrations (Ki = 2 µM).
Catalogue Number203291
Brand Family Calbiochem®
Synonyms2-[1-(3-Dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)maleimide, HCl
References
ReferencesLe Panse, R., et al. 1994. Mol. Pharmacol. 46, 445.
Kuchera, S., et al. 1993. Agents Actions 39, C169.
Wilkinson, S.E., et al. 1993. Biochem. J. 294, 335.
Toullec, D., et al. 1991. J. Biol. Chem. 266, 15771.
Product Information
CAS number176504-36-2
ATP CompetitiveY
FormOrange-red solid
Hill FormulaC₂₅H₂₄N₄O₂ · HCl
Chemical formulaC₂₅H₂₄N₄O₂ · HCl
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetProtein kinase C
Primary Target K<sub>i</sub>10 nM against protein kinase C
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
203291-1MG 04055977221152
203291-250UG 07790788048334

Documentation

Bisindolylmaleimide I, Hydrochloride - CAS 176504-36-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Bisindolylmaleimide I, Hydrochloride - CAS 176504-36-2 - Calbiochem Certificates of Analysis

TitleLot Number
203291

References

Reference overview
Le Panse, R., et al. 1994. Mol. Pharmacol. 46, 445.
Kuchera, S., et al. 1993. Agents Actions 39, C169.
Wilkinson, S.E., et al. 1993. Biochem. J. 294, 335.
Toullec, D., et al. 1991. J. Biol. Chem. 266, 15771.

Brochure

Title
PKC Pathway Poster PDF ( 676 KB )

Citations

Title
  • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
  • Michael J. Cross, et al. (2002) The Shb Adaptor Protein Binds to Tyrosine 766 in the FGFR-1 and Regulates the Ras/MEK/MAPK Pathway via FRS2 Phosphorylation in Endothelial Cells. Molecular Biology of the Cell 13, 2881-2893.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision08-January-2010 RFH
    Synonyms2-[1-(3-Dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)maleimide, HCl
    DescriptionA reversible and ATP-competitive inhibitor of protein kinase C (Ki = 10 nM). Inhibits protein kinase A at much higher concentrations (Ki = 2 µM). An enhanced water soluble form of Bisindolylmaleimide I (Cat. No. 203290).
    FormOrange-red solid
    CAS number176504-36-2
    Chemical formulaC₂₅H₂₄N₄O₂ · HCl
    Structure formulaStructure formula
    Purity≥95% by HPLC
    SolubilityDMSO (20 mg/ml) or H₂O (10 mg/ml)
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
    Toxicity Standard Handling
    ReferencesLe Panse, R., et al. 1994. Mol. Pharmacol. 46, 445.
    Kuchera, S., et al. 1993. Agents Actions 39, C169.
    Wilkinson, S.E., et al. 1993. Biochem. J. 294, 335.
    Toullec, D., et al. 1991. J. Biol. Chem. 266, 15771.
    Citation
  • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
  • Michael J. Cross, et al. (2002) The Shb Adaptor Protein Binds to Tyrosine 766 in the FGFR-1 and Regulates the Ras/MEK/MAPK Pathway via FRS2 Phosphorylation in Endothelial Cells. Molecular Biology of the Cell 13, 2881-2893.