Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on More

70746 Benzonase® Nuclease, Purity > 90%

View Products on
Purchase on Sigma-Aldrich


Replacement Information


Catalog NumberPackaging Qty/Pack
70746-3 Glass bottle 10 ku
70746-4 Plastic ampoule 2.5 ku
OverviewBenzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzonase to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster® and PopCulture® Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts.

The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.

Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity > 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied in 50% glycerol. Store at -20°C.

Total endotoxin: < 0.25 EU/1,000 units. Purity: > 90% by SDS-PAGE
Catalogue Number70746
Brand Family Novagen®
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
Quality LevelMQ300
Biological Information
PurityPurity: > 90% by SDS-PAGE.
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
70746-3 07790788052805
70746-4 04055977256949


Benzonase® Nuclease, Purity > 90% SDS


Safety Data Sheet (SDS) 

Benzonase® Nuclease, Purity > 90% Certificates of Analysis

TitleLot Number


Protein Purification and Detection Tools


  • Sandra Hervas-Stubbs, et al. (2007) Insect baculoviruses strongly potentiate adaptive immune responses by inducing type I IFN. Journal of Immunology 178, 2361-2369.
  • Samantha M. Lloyd-Burton, et al. (2007) Regulation of Ins(1,4,5)P3 3-kinases by calcium and localization in cells. Journal of Biological Chemistry 282, 9526-9535.
  • Federico Mingozzi, et al. (2007) Modulation of tolerance to the transgene product in a nonhuman primate model of AAV-mediated gene transfer to liver. Blood 110, 2334-2341.
  • Joao B. Oliveira, et al. (2007) NRAS mutation causes a human autoimmune lymphoproliferative syndrome. Procedings of the National Academy of Science 104, 8953-8958.
  • Myong-Ok Park, Taeko Mizutani and Patrik R. Jones. (2007) Glyceraldehyde-3-phosphate ferredoxin oxidoreductase from Methanococcus maripaludis. Journal of Bacteriology 189, 7281-7289.
  • Sehyung Cho, et al. (2005) Glucocorticoid receptor ligand binding domain is sufficient for the modulation of glucocorticoid induction properties by homologous receptors, coactivator transcription intermediary factor 2, and ubc9. Molecular Endocrinology 19, 290-311.
  • Paul Frankel, et al. (2005) RalA interacts with ZONAB in a cell density-dependent manner and regulates its transcriptional activity. European Molecular Biology Organization Journal 24, 54-62.
  • Weiping Han, et al. (2005) C-terminal ECFP Fusion Impairs Synaptotagmin 1 Function: crowding out synaptotagmin 1. Journal of Biological Chemistry 280, 5089-5100.
  • Line Johnsen, et al. (2005) 1.6 Å crystal structure of EntA-Im: A bacterial immunity protein conferring immunity to the antimicrobial activity of the pediocin-like bacteriocin enterocin A. Journal of Biological Chemistry 280, 19045-19050.
  • Sheila M. Keating, et al. (2005) Durable human memory T cells quantifiable by cultured enzyme-linked immunospot assays are induced by heterologous prime boost immunization and correlate with protection against malaria. Journal of Immunology 175, 5675-5680.
  • Desigan Kumaran, et al. (2005) Structure and mechanism of ADP-ribose-1''-monophosphatase (Appr-1''-pase), a ubiquitous cellular processing enzyme. Protein Science 14, 719-726.
  • Zuzanna Kwade, et al. (2005) Identification of four adenosine kinase isoforms in tobacco by-2 cells and their putative role in the cell cycle regulated cytokinin metabolism. Journal of Biological Chemistry 280, 17512-17519.
  • Laigeng Li, et al. (2005) Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen. Plant and Cell Physiology 46, 1073-1082.
  • Malcolm A. Meyn, et al. (2005) Src family kinase activity is required for murine embryonic stem cell growth and differentiation. Molecular Pharmacology 68, 1320-1330.
  • Michelle A. Poirier, Haibing Jiang and Christopher A. Ross. (2005) A structure-based analysis of huntingtin mutant polyglutamine aggregation and toxicity: evidence for a compact beta-sheet structure. Human Molecular Genetics 14, 765-774.
  • Jenni M. Vuola, et al. (2005) Differential immunogenicity of various heterologous prime-boost vaccine regimens using DNA and viral vectors in healthy volunteers. Journal of Immunology 174, 449-455.
  • Yasuo Yamakoshi, et al. (2005) Porcine dentin sialoprotein is a proteoglycan with glycosaminoglycan chains containing chondroitin 6-sulfate. Journal of Biological Chemistry 280, 1552-1560.
  • Christine N. Zanghi, et al. (2005) A simple method for displaying recalcitrant proteins on the surface of bacteriophage lambda. Nucleic Acids Research 33, e160.
  • Vivi Kasim, Makoto Miyagishi and Kazunari Taira. (2004) Control of siRNA expression using the Cre-loxP recombination system. Nucleic Acids Research 32, e66.
  • Vladimir V. Lunin, et al. (2004) The structure of the MAPK scaffold, MP1, bound to Its partner, p14 A complex with a critical role in endosomal MAP kinase signaling. Journal of Biological Chemistry 279, 23422-23430.
  • S. Moy, et al. (2004) Genome-scale expresion of proteins from Bacillus subtilis. Journal of Structural and Functional Genomics 5, 103-109.
  • Henry Nguyen, et al. (2004) An automated small-scale protein expression and purification screening provides beneficial information for protein production. Journal of Structural and Functional Genomics 5, 23-27.
  • Mark A. Rizzo, et al. (2004) An improved cyan fluorescent protein variant useful for FRET. Nature Biotechnology 22, 445-449.
  • Michael B. Murphy, et al. (2003) An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Research 31, e110-.
  • Wayne W. Chan, Steven L. Roderick and David E. Cohen. (2002) Human phosphatidylcholine transfer protein: purification, crystallization and preliminary X-ray diffraction data. Biochimica et Biophysica Acta 1596, 1-5.
  • Kaisa Huhtinen, et al. (2002) The peroxisome proliferator-induced cytosolic type I acyl-CoA thioesterase (CTE-I) is a serine-histidine-aspartic acid α/β hydrolase. Journal of Biological Chemistry 277, 3424-3432.
  • Leslie Lobel, et al. (2002) Bacterial expression of a natively folded extracellular domain fusion protein of the hFSH receptor in the cytoplasm of Escherichia coli. Protein Expression and Purification 25, 124-133.
  • Keri L.N. Mercer and David S. Weiss. (2002) The Escherichia coli cell divisionn protein FtsW is required to recruit its cognate transpeptidase, FtsI (PBP3), to the division site. Journal of Bacteriology 184, 904-912.
  • Michael Peitz, et al. (2002) Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: a tool for efficient genetic engineering of mammalian genomes. Procedings of the National Academy of Science 99, 4489-4494.
  • Tatiana Suarez, Subhasis B. Biswas and Esther E. Biswas. (2002) Biochemical defects in retina-specific human ATP binding cassette transporter nucleotide binding domain 1 mutants associated with macular degeneration. Journal of Biological Chemistry 277, 21759-21767.
  • Eitan Glick, Kellie L. Vigna and Lawrence A. Loeb. (2001) Mutations in human DNA polymerase η motif II after bypass of DNA lesions. European Molecular Biology Organization Journal 20, 7303-7312.
  • Marcel Hoffman, et al. (2001) Differential capacities of the RGS1, RGS16 and RGS-GAIP regulators of G protein signaling to enhance α2A-adrenoreceptor agonist-stimulated GTPase activity of Go1α. Journal of Neurochemistry 78, 797-806.
  • L.I. Lobel, et al. (2001) High-level bacterial expression of a natively folded, soluble extracellular domain fusion protein of the human luteinizing hormaone/ chorionic gonadotropin receptor in the cytoplasm of Escherichia coli. Endocrine 14, 205-212.
  • Sujata Persad, et al. (2001) Regulation of protein kinase B/ Akt-serine 473 phosphorylation by integrin-linked kinase. Journal of Biological Chemistry 276, 27462-27469.
  • User Protocols

    TB261 Benzonase® Nuclease