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Description
Overview
Recombinant, human bone morphogenetic protein-2 (BMP-2) expressed in E. coli and purified by proprietary chromatographic methods. BMP-2 is a homodimeric protein that belongs to the transforming growth factor-β (TGF-β) superfamily. BMP-2 is known to regulate diverse fundamental processes during embryonic development. It also plays an important role in cardiac morphogenesis and is expressed in a variety of tissues such as lung, spleen, brain, liver, prostate, ovary, and small intestine.
Catalogue Number
203641
Brand Family
Calbiochem®
Synonyms
Bone morphogenetic protein 2 precursor, BMP-2A
References
References
Csiszar, A., et al. 2006. Am. J. Pathol.168, 629. Kumagai, T., et al. 2006. Anticancer Res.26, 293. Kumagai, T., et al. 2006. Int. J. Mol. Med.17, 285. Kuo, P.L., et al. 2006. Biol. Pharm. Bull.29, 119. Morin, R., et al. 2006. Biomacromolecules7, 131. Villavicencio, A.T., et al. 2005. J. Neurosurg. Spine3, 436.
Product Information
Form
Lyophilized
Formulation
Lyophilized from sterile 10 mM sodium citrate, pH 3.5.
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for 2-7 days at 4°C or for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
BMP-2, Human, Recombinant, E. coli Certificates of Analysis
Title
Lot Number
203641
References
Reference overview
Csiszar, A., et al. 2006. Am. J. Pathol.168, 629. Kumagai, T., et al. 2006. Anticancer Res.26, 293. Kumagai, T., et al. 2006. Int. J. Mol. Med.17, 285. Kuo, P.L., et al. 2006. Biol. Pharm. Bull.29, 119. Morin, R., et al. 2006. Biomacromolecules7, 131. Villavicencio, A.T., et al. 2005. J. Neurosurg. Spine3, 436.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
20-May-2008 RFH
Synonyms
Bone morphogenetic protein 2 precursor, BMP-2A
Description
Recombinant, human bone morphogenetic protein-2 (BMP-2) expressed in E. coli and purified by proprietary chromatographic methods. BMP-2 is a homodimeric protein that belongs to the transforming growth factor-β (TGF-β) superfamily. BMP-2 is known to regulate diverse fundamental processes during embryonic development. It also plays an important role in cardiac morphogenesis and is expressed in a variety of tissues such as lung, spleen, brain, liver, prostate, ovary, and small intestine.
Form
Lyophilized
Formulation
Lyophilized from sterile 10 mM sodium citrate, pH 3.5.
Purity
≥95% by SDS-PAGE
Solubility
Reconstitute to ≥100 µg/ml with 20 mM acetic acid containing 0.1% HSA or BSA. Further dilutions can be made in aqueous buffers just prior to use.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for 2-7 days at 4°C or for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Csiszar, A., et al. 2006. Am. J. Pathol.168, 629. Kumagai, T., et al. 2006. Anticancer Res.26, 293. Kumagai, T., et al. 2006. Int. J. Mol. Med.17, 285. Kuo, P.L., et al. 2006. Biol. Pharm. Bull.29, 119. Morin, R., et al. 2006. Biomacromolecules7, 131. Villavicencio, A.T., et al. 2005. J. Neurosurg. Spine3, 436.