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WB57 BLOT-QuickBlocker™ Reagent

WB57
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
WB57-175GM Plastic ampoule 175 gm
Description
OverviewA unique modified milk protein that can be used as a blocking agent in Western blots, dot blots and ELISA, including dilution of primary and secondary antibodies. In contrast to dry milk powder, BLOT-QuickBlocker™ Reagent is a semi-clear 2-5% solution when dissolved in PBST or TBST, allowing for examination of blot membranes during the blotting procedure. BLOT-QuickBlocker™ Reagent has a high blocking efficiency, blocks within 15-20 minutes, produces a clear background, and does not inhibit peroxidase.
Catalogue NumberWB57
Brand Family Calbiochem®
References
Product Information
FormSolid
FormulationSupplied as modified milk protein.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application CommentsADVANTAGES
• Readily soluble and produces a stable solution that can be reused
• Fat free
• Does not inhibit peroxidase
• Produces clear background
• Offers higher blocking efficiency
• Blocking time is 15-60 min
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsPrepare a 2-5% solution in PBST or TBST. Incubate for 15-60 min with membrane prior to staining with antibody.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
WB57-175GM 04055977206739

Documentation

BLOT-QuickBlocker™ Reagent Certificates of Analysis

TitleLot Number
WB57
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-August-2008 JSW
BackgroundA unique modified milk protein that can be used as a blocking agent in Western blots, dot blots and ELISA, including dilution of primary and secondary antibodies. In contrast to dry milk powder, BLOT-QuickBlocker™ Reagent is a semi-clear 2-5% solution when dissolved in TBST or PBST, allowing for examination of blot membranes during the blotting procedure. BLOT-QuickBlocker™ Reagent has a high blocking efficiency, blocks within 15-20 minutes, produces a clear background, and does not inhibit peroxidase.
FormSolid
FormulationSupplied as modified milk protein.
PreservativeNone
CommentsADVANTAGES
• Readily soluble and produces a stable solution that can be reused
• Fat free
• Does not inhibit peroxidase
• Produces clear background
• Offers higher blocking efficiency
• Blocking time is 15-60 min
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsPrepare a 2-5% solution in PBST or TBST. Incubate for 15-60 min with membrane prior to staining with antibody.
Toxicity Standard Handling