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Description
Overview
A unique modified milk protein that can be
used as a blocking agent in Western blots, dot blots and ELISA, including dilution of primary and secondary antibodies. In contrast to dry milk powder, BLOT-QuickBlocker™ Reagent is a semi-clear 2-5% solution when dissolved in PBST or TBST, allowing for examination of blot membranes during the blotting procedure. BLOT-QuickBlocker™ Reagent has a high blocking efficiency, blocks within 15-20 minutes, produces a clear background, and does not inhibit peroxidase.
ADVANTAGES • Readily soluble and produces a stable solution that can be reused • Fat free • Does not inhibit peroxidase • Produces clear background • Offers higher blocking efficiency • Blocking time is 15-60 min
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+15°C to +30°C
Do not freeze
Ok to freeze
Special Instructions
Prepare a 2-5% solution in PBST or TBST. Incubate for 15-60 min with membrane prior to staining with antibody.
Packaging Information
Transport Information
Supplemental Information
Specifications
Documentation
BLOT-QuickBlocker™ Reagent Certificates of Analysis
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
13-August-2008 JSW
Background
A unique modified milk protein that can be
used as a blocking agent in Western blots, dot blots and ELISA, including dilution of primary and secondary antibodies. In contrast to dry milk powder, BLOT-QuickBlocker™ Reagent is a semi-clear 2-5% solution when dissolved in TBST or PBST, allowing for examination of blot membranes during the blotting procedure. BLOT-QuickBlocker™ Reagent has a high blocking efficiency, blocks within 15-20 minutes, produces a clear background, and does not inhibit peroxidase.
Form
Solid
Formulation
Supplied as modified milk protein.
Preservative
None
Comments
ADVANTAGES • Readily soluble and produces a stable solution that can be reused • Fat free • Does not inhibit peroxidase • Produces clear background • Offers higher blocking efficiency • Blocking time is 15-60 min
Storage
+15°C to +30°C
Do Not Freeze
Ok to freeze
Special Instructions
Prepare a 2-5% solution in PBST or TBST. Incubate for 15-60 min with membrane prior to staining with antibody.
