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69041 B834(DE3) Competent Cells - Novagen

69041
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          Description
          OverviewB834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2).

          DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.



          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Genotype: F- ompT hsdSB(rB- mB-) gal dcm met (DE3)


          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number69041
          Brand Family Novagen®
          References
          References1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

          2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
          Product Information
          Components
          Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁶ cfu/μg
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          B834(DE3) Competent Cells - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          B834(DE3) Competent Cells - Novagen Certificates of Analysis

          TitleLot Number
          69041

          References

          Reference overview
          1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

          2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.

          Citations

          Title
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        • Alderwick, L.J., et al. 2006. Proceedings of the National Academy of Sciences (USA) 103, 2558.
        • Chédin, F., et al. 2006. Journal of Biological Chemistry 281, 18610.
        • Sasaki, H.M., et al. 2006. Procedings of the National Academy of Science 103, 14744.
        • Sheckler, L.R., et al. 2006. Journal of Biological Chemistry 281, 22896.
        • Song, F., et al. 2006. Journal of Biological Chemistry 281, 11028.
        • Alphey, M.S., et al. 2005. Journal of Biological Chemistry 280, 3068.
        • Dey, S., et al. 2005. Journal of Biological Chemistry 280, 14892.
        • Maria- Eugenia Guazzaroni, M-F., et al. 2005. Journal of Biological Chemistry 280, 20887.
        • Jogl, G., et al. 2005. i>Journal of Biological Chemistry 280, 738.
        • Li, H., et al. 2005. Protein Science 14, 3025.
        • Li, K., et al. 2005. Journal of Biological Chemistry 280, 29176.
        • Singh, S., et al. 2005. Journal of Biological Chemistry 280, 17101.
        • Zhu, G., et al. 2005. Journal of Biological Chemistry 280, 23057.
        • Chen, Y-h., et al. 2004. Nature. 429, 675.
        • Hsieh, T-J., et al. 2004. Journal of Biological Chemistry 279, 55587.
        • Lehmann, C., et al. 2004. Proteins: Structure, Function, and Bioinformatics 56, 376.
        • Mancini, E.J., et al. 2004. Acta Crystallographica D60, 588.
        • Nguyen, H., et al. 2004. Journal of Structural and Functional Genomics 5, 23.
        • James S. Parker, S. Mark Roe and David Barford. 2004. European Molecular Biology Organization Journal 23, 4727.
        • Welin, M., et al. 2004. Procedings of the National Academy of Science 101, 17970.
        • Obmolova, G., et al. 2000. Nature 407, 703.
        • David K. Worthylake, Kent L. Rossman and John Sondek. 2000. Nature 408, 682.
        • Conn, G.L., et al. 1999. Science 284, 1171.
        • Joey M. Studts and Brian G, Fox. 1999. Protein Expression and Purification 16, 109.
        • Cabral, J.H.M., et al. 1998. Cell 95, 649.
        • Fauman, E.B., et al. 1998. Cell 93, 617.
        • Gomis-Ruth, F.-X., et al. 1997. Nature 389, 77.
        • Dyda, F., et al. 1994. Science 266, 1981.
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