182515 Sigma-AldrichArp2/3 Complex Inhibitor I, CK-666 - CAS 442633-00-3 - Calbiochem
Arp2/3 Complex Inhibitor I, CK-666, CAS 442633-00-3, is a cell-permeable selective inhibitor of actin assembly mediated by actin-related protein Arp2/3 complex (IC50 = 4 uM in human).
More>> Arp2/3 Complex Inhibitor I, CK-666, CAS 442633-00-3, is a cell-permeable selective inhibitor of actin assembly mediated by actin-related protein Arp2/3 complex (IC50 = 4 uM in human). Less<<Synonyms: 2-Fluoro-N-(2-(2-Methyl-1H-indol-3-yl)ethyl)benzamide, CK-0944666, Actin Assembly Inhibitor XIII
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Overview
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Key Specifications Table
CAS # | Empirical Formula |
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442633-00-3 | C₁₈H₁₇FN₂O |
Products
Catalog Number | Packaging | Qty/Pack | |
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182515-25MG | Glass bottle | 25 mg |
Description | |
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Overview | A cell-permeable indolyl-fluorobenzamide compound that selectively inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human, bovine, fission yeast S. pombe, and budding yeast S. cerevisiae origin (IC50 = 4, 17, 5, and 12 µM, respectively), without affecting S. pombe formin domain Cdc12(FH2)-mediated or the spontaneous actin polymerization. Shown to inhibit the actin filament "comet tails" formation around intracellular Listeria in infected SKOV3 cells (IC50 = 7 µM) in a reversible manner. Structrual studies indicate that CK-666 targets a pocket formed between subdomain 4 of Arp2 and subdomain 1 of Arp3, preventing Arp2/3 from shifting into an active conformation upon N-WASP-VCA binding. CK-666 and CK-869 (Cat. No. 182516) exhibit different modes of binding, resulting in their different yeast cross-reactivities. CK-689 (Cat. No. 182517) can serve as a negative control. |
Catalogue Number | 182515 |
Brand Family | Calbiochem® |
Synonyms | 2-Fluoro-N-(2-(2-Methyl-1H-indol-3-yl)ethyl)benzamide, CK-0944666, Actin Assembly Inhibitor XIII |
Application Data |
References | |
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References | Nolen, B.J., et al. 2009. Nature 460, 1031. |
Product Information | |
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CAS number | 442633-00-3 |
Form | Off-white solid |
Hill Formula | C₁₈H₁₇FN₂O |
Chemical formula | C₁₈H₁₇FN₂O |
Quality Level | MQ100 |
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Purity | ≥95% by HPLC |
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Packaged under inert gas | Packaged under inert gas |
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Specifications |
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Global Trade Item Number | |
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Catalog Number | GTIN |
182515-25MG | 04055977205053 |
Documentation
Arp2/3 Complex Inhibitor I, CK-666 - CAS 442633-00-3 - Calbiochem SDS
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Arp2/3 Complex Inhibitor I, CK-666 - CAS 442633-00-3 - Calbiochem Certificates of Analysis
Title | Lot Number |
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182515 |
References
Reference overview |
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Nolen, B.J., et al. 2009. Nature 460, 1031. |