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508774 Apoptosis Activator VIII, TP421 - Calbiochem

A cell-permeable inhibitor of cancer cell proliferation (IC₅₀ = 400, 500, 800 nM HPAC, MIA, and PaCa-2 cells, respectively). Arrests cell cycle at G0/G1 phase and reduces autophagy.

Apoptosis Activator VIII, TP421 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (2-(7-(Diethylamino)-4-methyl-2-oxo-2H-chromen-3-yl)ethyl)triphenylphosphonium Salt

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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₃₄H₃₅NO₂P+Br-.H₂O

Products

Catalog NumberPackaging Qty/Pack
5.08774.0001 Glass bottle 10 mg
Description
OverviewA cell-permeable triphenylphosphonium compound that exhibits selective toxicity towards cancer cells and inhibits their proliferation (IC50 = 400, 500, 800 nM and 1.1. µM for HPAC, MIA PaCa-2, BxPC-3, and PANC-1 pancreatic cancer cells, respectively). Arrests cell cycle at G0/G1 phase of the cell cycle in a time- and dose-dependent manner. It accumulates in mitochondria and induces mitochondrial and cytosolic accumulation of reactive oxygen species. Induces apoptosis by activating caspase-8 and 7 and by lowering the levels of Bcl-2 and survivin in cancer cells. Reduces autophagy as evidenced by a reduction in beclin-1 and increase in LC3B-II and p62 levels (at ~20 µM). Also shown to diminish Src phosphorylation (Tyr416) correspondingly reduce in Ser576 and Tyr861 phosphorylation in focal adhesion kinase (FAK).

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number508774
Brand Family Calbiochem®
Synonyms(2-(7-(Diethylamino)-4-methyl-2-oxo-2H-chromen-3-yl)ethyl)triphenylphosphonium Salt
References
ReferencesMillard, M., et al. 2013. PLos One. 5, e13131.
Product Information
FormYellow powder
Hill FormulaC₃₄H₃₅NO₂P+Br-.H₂O
Chemical formulaC₃₄H₃₅NO₂P+Br-.H₂O
Quality LevelMQ100
Applications
Biological Information
Primary Target IC<sub>50</sub>400 nM
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Identification Number
Catalog Number GTIN
5.08774.00014055977261400

Documentation

Apoptosis Activator VIII, TP421 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Millard, M., et al. 2013. PLos One. 5, e13131.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-April-2014 JSW
Synonyms(2-(7-(Diethylamino)-4-methyl-2-oxo-2H-chromen-3-yl)ethyl)triphenylphosphonium Salt
DescriptionA cell-permeable triphenylphosphonium compound that exhibits selective toxicity towards cancer cells and inhibits their proliferation (IC50 = 400, 500, 800 nM and 1.1. µM for HPAC, MIA PaCa-2, BxPC-3, and PANC-1 pancreatic cancer cells, respectively). Arrests cell cycle at G0/G1 phase of the cell cycle in a time- and dose-dependent manner. It accumulates in mitochondria and induces mitochondrial and cytosolic accumulation of reactive oxygen species. Induces apoptosis by activating caspase-8 and 7 and by lowering the levels of Bcl-2 and survivin in cancer cells. Reduces autophagy as evidenced by a reduction in beclin-1 and increase in LC3B-II and p62 levels (at ~20 µM). Also shown to diminish Src phosphorylation (Tyr416) correspondingly reduce in Ser576 and Tyr861 phosphorylation in focal adhesion kinase (FAK).
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₃₄H₃₅NO₂P+Br-.H₂O
Purity≥98% by HPLC
SolubilityDMSO (100 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesMillard, M., et al. 2013. PLos One. 5, e13131.