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05-368 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody

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05-368
200 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtELISA, IHC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-368
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Ser/Thr-Pro MPM-2 Antibody
OverviewThis antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Background InformationUpon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
References
Product Information
FormatPurified
Control
  • Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde
PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Ser/Thr-Pro MPM-2 Antibody is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in ELISA, IHC, IP & WB.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Biological Information
ImmunogenMitotic human HeLa cell cytosolic lysate
CloneMPM-2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes phosphorylated serines or threonines when followed by a proline.
IsotypeIgG1
Species Reactivity
  • Vertebrates
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
Molecular Weightvaries depending on the protein you are detecting
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-368 04053252371424

Documentation

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody Certificates of Analysis

TitleLot Number
-3156757 3156757
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 4061115
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 2424761 2424761
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 2452516 2452516
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 16516 16516
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 16648 16648
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 16781 16781
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 18594 18594
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 1992443 1992443
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 20758 20758

References

Reference overviewApplicationSpeciesPub Med ID
Dual targeting of microtubule and topoisomerase II by α-carboline derivative YCH337 for tumor proliferation and growth inhibition.
Yi, JM; Zhang, XF; Huan, XJ; Song, SS; Wang, W; Tian, QT; Sun, YM; Chen, Y; Ding, J; Wang, YQ; Yang, CH; Miao, ZH
Oncotarget  6  8960-73  2015

Show Abstract
25840421 25840421
FBXW7 and USP7 regulate CCDC6 turnover during the cell cycle and affect cancer drugs susceptibility in NSCLC.
Morra, F; Luise, C; Merolla, F; Poser, I; Visconti, R; Ilardi, G; Paladino, S; Inuzuka, H; Guggino, G; Monaco, R; Colecchia, D; Monaco, G; Cerrato, A; Chiariello, M; Denning, K; Claudio, PP; Staibano, S; Celetti, A
Oncotarget  6  12697-709  2015

Show Abstract
25885523 25885523
Multiple assembly mechanisms anchor the KMN spindle checkpoint platform at human mitotic kinetochores.
Kim, S; Yu, H
The Journal of cell biology  208  181-96  2015

Show Abstract
25601404 25601404
Pin1-dependent signalling negatively affects GABAergic transmission by modulating neuroligin2/gephyrin interaction.
Antonelli, R; Pizzarelli, R; Pedroni, A; Fritschy, JM; Del Sal, G; Cherubini, E; Zacchi, P
Nature communications  5  5066  2014

Show Abstract
25297980 25297980
Protein kinase C β inhibition by enzastaurin leads to mitotic missegregation and preferential cytotoxicity toward colorectal cancer cells with chromosomal instability (CIN).
Ouaret, D; Larsen, AK
Cell cycle (Georgetown, Tex.)  13  2697-706  2014

Show Abstract
25486357 25486357
Identification of a mitotic death signature in cancer cell lines.
Sakurikar, N; Eichhorn, JM; Alford, SE; Chambers, TC
Cancer letters  343  232-8  2014

Show Abstract
24099917 24099917
Homeostatic control of polo-like kinase-1 engenders non-genetic heterogeneity in G2 checkpoint fidelity and timing.
Liang, H; Esposito, A; De, S; Ber, S; Collin, P; Surana, U; Venkitaraman, AR
Nature communications  5  4048  2014

Show Abstract
Immunofluorescence24893992 24893992
Orally active microtubule-targeting agent, MPT0B271, for the treatment of human non-small cell lung cancer, alone and in combination with erlotinib.
Tsai, AC; Wang, CY; Liou, JP; Pai, HC; Hsiao, CJ; Chang, JY; Wang, JC; Teng, CM; Pan, SL
Cell death & disease  5  e1162  2014

Show Abstract
24722287 24722287
Comparative analysis of mitosis-specific antibodies for bulk purification of mitotic populations by fluorescence-activated cell sorting.
Campbell, Amy E, et al.
BioTechniques, 56: 90-4 (2014)  2014

Show Abstract
Flow Cytometry24502799 24502799
Small molecule R1498 as a well-tolerated and orally active kinase inhibitor for hepatocellular carcinoma and gastric cancer treatment via targeting angiogenesis and mitosis pathways.
Zhang, C; Wu, X; Zhang, M; Zhu, L; Zhao, R; Xu, D; Lin, Z; Liang, C; Chen, T; Chen, L; Ren, Y; Zhang, J; Qin, N; Zhang, X
PloS one  8  e65264  2013

Show Abstract
Immunofluorescence23755206 23755206

Related Products & Applications

Alternative Packsize

Catalog Number Description
05-368-KC Anti-phospho-Ser/Thr-Pro, MPM-2 (KC)
05-368MG Anti-phospho-Ser/Thr-Pro MPM-2 Antibody

Alternative Format

Catalog Number Description
16-155 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, FITC conjugate
16-220 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, Cy5 conjugate

Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies