07-1348 Anti-phospho-PLK1 (Ser137) Antibody

07-1348
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-1348
      DescriptionAnti-phospho-PLK1 (Ser137) Antibody
      Alternate Names
      • Polo-like kinase 1
      • Serine/threonine-protein kinase 13
      • cell cycle regulated protein kinase
      • polo (Drosophia)-like kinase
      • polo like kinase
      • polo-like kinase (Drosophila)
      • polo-like kinase 1 (Drosophila)
      Background InformationPolo-like Kinase 1 (PLK1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Lambda phosphatase treated and untreated human placenta tissue lysate.
      PresentationAffinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect phospho-PLK1 (Ser137) using this Anti-phospho-PLK1 (Ser137) Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (SNAP ID) Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in human placenta tissue lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phosphoylated PLK1 at and around Ser137.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes phosphorylated PLK1 at Ser137.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      Species Reactivity NoteHuman, Rat and Mouse. Predicted to react with Cow based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EC 2.7.11.21
      • PLK
      • PLK-1
      • STPK13
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      ENZYME REGULATION: Activated by serine and threonine phosphorylation.
      SUBUNIT STRUCTURE: Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L. Interacts with FAM29A. Interacts with BTBD12/SLX4. Ref.11 Ref.12 Ref.13 Ref.14 Ref.18
      SUBCELLULAR LOCATION:Nucleus.
      TISSUE SPECIFICITY:Placenta and colon.
      DEVELOPMENTAL STAGE:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
      INDUCTION: By growth-stimulating agents.
      PTM: Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137.Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
      Contains 2 POLO box domains.
      Contains 1 protein kinase domain.
      Molecular Weight68 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human placenta tissue lysate.
      Western Blot Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in lambda phosphatase treated and untreated human placenta tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-PLK1 (Ser137) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-PLK1 (Ser137) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-PLK1 (Ser137) - 21470982147098
      Anti-phospho-PLK1 (Ser137) - 24334402433440
      Anti-phospho-PLK1 (Ser137) - 20631852063185
      Anti-phospho-PLK1 (Ser137) - 22778922277892
      Anti-phospho-PLK1 (Ser137) - 23306142330614
      Anti-phospho-PLK1 (Ser137) - 33073013307301
      Anti-phospho-PLK1 (Ser137) - NG1726976NG1726976
      Anti-phospho-PLK1 (Ser137) - NG1857492NG1857492
      Anti-phospho-PLK1 (Ser137) - NG1863946NG1863946
      Anti-phospho-PLK1 (Ser137) - NRG1652822NRG1652822

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition (MilliporeSigma)