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ABS45 Anti-phospho-MYPT1 (Thr696) Antibody

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Pm, R, Ca, Xn, Eq, ChWBRbAffinity PurifiedPolyclonal Antibody
      Catalogue NumberABS45
      DescriptionAnti-phospho-MYPT1 (Thr696) Antibody
      Alternate Names
      • Myosin phosphatase target subunit 1
      • Myosin phosphatase-targeting subunit 1
      • Protein phosphatase myosin-binding subunit
      • myosin phosphatase, target subunit 1
      • protein phosphatase 1, regulatory (inhibitor) subunit 12
      Background InformationMyosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. It is also involved in smooth muscle contraction. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1. PP1c interacts with at least four binding sites on the amino-terminus of MYPT1. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity.
      Product Information
      FormatAffinity Purified
      • Calyculin/Okadaic treated and untreated HEK293 cell lysates
      PresentationPurified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-phospho-MYPT1 (Thr696) Antibody is an antibody against phospho-MYPT1 (Thr696) for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting (SNAP ID)Analysis: 0.1 µg/mL from a previous lot detected MYPT1 on 10 µg of NIH/3T3 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phosphorylated Thr696 of MYPT1.
      EpitopePhosphorylated Thr696
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes phosphorylated MYPT1 at Thr696.
      Species Reactivity
      • Human
      • Mouse
      • Primate
      • Rat
      • Canine
      • Xenopus
      • Horse
      • Chicken
      Species Reactivity NoteReacts with human and mouse. Predicted to react with primate, dog, rat, xenopus, horse, chicken, cow, monkey, and danio based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMyosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small
      guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells.The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of
      phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA,
      phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • MBS
      • MGC133042
      • MYPT1
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Regulates myosin phosphatase activity.
      SUBUNIT STRUCTURE: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT By similarity. Binds PPP1R12B, ROCK1 and IL16.
      SUBCELLULAR LOCATION: Cytoplasm. Note: Along actomyosin filaments and stress fibers.
      PTM: Phosphorylated by CIT (Rho-associated kinase) By similarity. Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      SEQUENCE SIMILARITIES: Contains 6 ANK repeats.
      Molecular WeightExpected at 115 kDa and observed at 130 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.
      Western Blotting Analysis: 0.1 µg/mL of this antibody was used to detect MYPT1 phosphorylated at Thr696 on 10 µg of Calyculin/Okadaic treated and untreated HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information


      Anti-phospho-MYPT1 (Thr696) Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-phospho-MYPT1 (Thr696) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-MYPT1 (Thr696) - 2121780 2121780
      Anti-phospho-MYPT1 (Thr696) - 2377421 2377421
      Anti-phospho-MYPT1 (Thr696) - 2452524 2452524
      Anti-phospho-MYPT1 (Thr696) - 2181279 2181279
      Anti-phospho-MYPT1 (Thr696) - 2290259 2290259
      Anti-phospho-MYPT1 (Thr696) - 2476996 2476996
      Anti-phospho-MYPT1 (Thr696) - 3194632 3194632
      Anti-phospho-MYPT1 (Thr696) - 3383154 3383154
      Anti-phospho-MYPT1 (Thr696) - 3610571 3610571
      Anti-phospho-MYPT1 (Thr696) - 3733837 3733837


      Reference overviewPub Med ID
      Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.
      Robertson, J; Jacquemet, G; Byron, A; Jones, MC; Warwood, S; Selley, JN; Knight, D; Humphries, JD; Humphries, MJ
      Nature communications  6  6265  2015

      Show Abstract
      25677187 25677187
      Inactivation of the Hippo tumour suppressor pathway by integrin-linked kinase.
      Serrano, I; McDonald, PC; Lock, F; Muller, WJ; Dedhar, S
      Nature communications  4  2976  2013

      Show Abstract
      24356468 24356468
      MRP4-mediated regulation of intracellular cAMP and cGMP levels in trabecular meshwork cells and homeostasis of intraocular pressure.
      Pattabiraman, PP; Pecen, PE; Rao, PV
      Investigative ophthalmology & visual science  54  1636-49  2013

      Show Abstract
      23385799 23385799
      Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment.
      Shi, J; Wu, X; Surma, M; Vemula, S; Zhang, L; Yang, Y; Kapur, R; Wei, L
      Cell death & disease  4  e483  2013

      Show Abstract
      23392171 23392171
      The role of calcium-independent phospholipase A2γ in modulation of aqueous humor drainage and Ca2+ sensitization of trabecular meshwork contraction.
      Pattabiraman, PP; Lih, FB; Tomer, KB; Rao, PV
      American journal of physiology. Cell physiology  302  C979-91  2012

      Show Abstract
      22237407 22237407
      RKI-1447 is a potent inhibitor of the Rho-associated ROCK kinases with anti-invasive and antitumor activities in breast cancer.
      Patel, RA; Forinash, KD; Pireddu, R; Sun, Y; Sun, N; Martin, MP; Schönbrunn, E; Lawrence, NJ; Sebti, SM
      Cancer research  72  5025-34  2012

      Show Abstract
      22846914 22846914
      Mechanisms underlying potentiation of endothelin-1-induced myofilament Ca(2+) sensitization after subarachnoid hemorrhage.
      Kikkawa, Y; Matsuo, S; Kameda, K; Hirano, M; Nakamizo, A; Sasaki, T; Hirano, K
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  32  341-52  2012

      Show Abstract
      21952110 21952110
      Rho-kinase inhibition alleviates pulmonary hypertension in transgenic mice expressing a dominant-negative type II bone morphogenetic protein receptor gene.
      Yasuda, T; Tada, Y; Tanabe, N; Tatsumi, K; West, J
      American journal of physiology. Lung cellular and molecular physiology  301  L667-74  2011

      Show Abstract
      21856816 21856816

      Technical Info

      Protein Blotting Handbook: 6th Edition

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      Life Science Research > Antibodies and Assays > Primary Antibodies