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05-807 Anti-phospho-CREB (Ser133) Antibody, clone 634-2

05-807
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RICC, IF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-807
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-CREB (Ser133) Antibody, clone 634-2
      Alternate Names
      • active transcription factor CREB
      • cAMP responsive element binding protein 1
      • cAMP-response element-binding protein-1
      • transactivator protein
      Background InformationCREB is a β ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.
      References
      Product Information
      FormatPurified
      Control
      • Human breast carcinoma, forskolin- and FGF-treated SK-N-MC cell extracts.
      PresentationPurified mouse IgG1κ in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20ºC.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-phospho-CREB (Ser133) Antibody, clone 634-2 (mouse monoclonal antibody) validated in ICC, IF, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesBeadlyte Specificity Assay:
      1:500 to 1:32,000 dilutions of this lot detected CREB peptide containing phosphoserine 133. The antibody did NOT recognize the nonphosphorylated peptide of the same sequence

      Immunofluorescence / Immunocytochemistry:
      This antibody has been reported by an independent laboratory to detect phospho-CREB (Ser133) using immunofluorescence.
      Biological Information
      ImmunogenA proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.
      EpitopeSer133
      Clone634-2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityPhospho-CREB (Ser133).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse and rat. Broad species cross-reactivity expected, based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
      Gene Symbol
      • CREB
      • MGC9284
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.
      SIZE: 341 amino acids; 36688 Da
      SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.
      Molecular Weight43 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells.

      Western Blot Analysis:
      A 1:1,000 to 1:4,000 dilution of this lot detected phospho-CREB (Ser 133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells (Figure A).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-CREB (Ser133) Antibody, clone 634-2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-CREB (Ser133) Antibody, clone 634-2 Certificates of Analysis

      TitleLot Number
      Anti-phospho-CREB (Ser133), clone 634-2 3074934
      Anti-phospho-CREB (Ser133), clone 634-2 - 2113352 2113352
      Anti-phospho-CREB (Ser133), -2581312 2581312
      Anti-phospho-CREB (Ser133), -2645946 2645946
      Anti-phospho-CREB (Ser133), -2685232 2685232
      Anti-phospho-CREB (Ser133), -2736610 2736610
      Anti-phospho-CREB (Ser133), -2806082 2806082
      Anti-phospho-CREB (Ser133), clone 634-2 - 2194160 2194160
      Anti-phospho-CREB (Ser133), clone 634-2 - 2361835 2361835
      Anti-phospho-CREB (Ser133), clone 634-2 - 26924 26924

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies