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05-667 Anti-phospho-CREB (Ser133) Antibody, clone 10E9

05-667
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, CaWB, ELISA, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-667
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-CREB (Ser133) Antibody, clone 10E9
      Alternate Names
      • active transcription factor CREB
      • cAMP responsive element binding protein 1
      • cAMP-response element-binding protein-1
      • transactivator protein
      Background InformationCyclic AMP-responsive element-binding protein 1 (UniProt: P16220; also known as CREB-1, cAMP-responsive element-binding protein 1) is encoded by the CREB1 gene (Gene ID: 1385) in human. CREB is a phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE; TGACGTCA) as a dimer. It is essential for the activation of a number of immediate early genes. It also plays a key role in promoting neuronal survival, differentiation, proliferation, and neurite outgrowth. Transcription activation is reported to be enhanced by the TORC coactivators. CREB has four main functional domains: a C-terminal basic/leucine zipper domain (bZIP; aa 269-327), the Kinase Inducible Domain at the N-terminal (KID; aa 87-146), and two glutamine-rich domains (Q1, and Q2). The Q1 and Q2 domains provide transactivation function in the absence of cAMP signaling and synergize with KID and bZIP to confer full activity to CREB in response to cAMP. CREB is phosphorylated on serine 133 by protein kinase A, which promotes the recruitment of the co-activator CREB-binding protein(CBP) and p300 that increases the transcription of CREB-dependent genes. CREB is also phosphorylated on serine 133 by mitogen- and stress-activated kinase 1/2 (MASK 1/2) in cells in response to the activation of MAPK signaling. Phosphorylation of CREB is sufficient to induce cellular gene expression in response to cAMP, however, additional promoter-bound factors are required for target gene activation by CREB in response to mitogen or stress signals, indicating the presence of components in the CREB pathway that discriminate between different types of inputs. (Ref.: Wang, H., et al. (2018). Front. Mol. Neurosci. 11; 255; Naqvi, S., et al. (2014) Biochem. J. 458(3); 469-479; Mayr, B., and Montiminy, M. (2001). Nat. Rev. Mol. Cell Biol. 2(8); 599-609).
      References
      Product Information
      FormatPurified
      Control
      • Forskolin-treated PC12 cell lysate
      PresentationPurified mouse monoclonal IgG1κ from serum-free cell culture supernatant, lyophilized from 1 mL of PBS, 0.09% sodium azide, PEG, and sucrose. Lyophilized.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-CREB (Ser133) Antibody, clone 10E9 is a Mouse Monoclonal Antibody for detection of phospho-CREB (Ser133) also known as active transcription factor CREB & has been validated in WB, ELISA & Immunohistochemistry.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry
      Application NotesELISA: Recommended at 0.05 μg/mL by an independent laboratory.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (REILSRRP(pS)YR) corresponding to amino acids 125-135 of human CREB. The immunizing sequence is identical in human and mouse. Clone 10E9.
      Cloneclone 10E9
      HostMouse
      SpecificityRecognizes phosphorylated CREB at Mr 46 kDa. May also recognize p30 and p38 proteins, which likely correspond to phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology with the phosphorylated CREB immunizing peptide
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Canine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
      Gene Symbol
      • CREB1
      • CREB
      • MGC9284
      Modifications
      • Phosphorylation
      Purification MethodThiophilic adsoption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.
      SIZE: 341 amino acids; 36688 Da
      SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.
      Molecular Weight46 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on whole cells lysates from rat PC12 cells treated with forskolin.

      Western Blot Analysis: 0.5-2 μg/mL of this antibody detected phosphorylated CREB in whole cell lysates from HepG2 and PC12 cells treated with forskolin.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLyophilized: Stable for 1 year at -20ºC from date of receipt.
      Rehydrated: Stable for 3 months at 2-8ºC.
      Aliquot solution to store frozen and avoid repeated freeze thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-CREB (Ser133) Antibody, clone 10E9 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-CREB (Ser133) Antibody, clone 10E9 Certificates of Analysis

      TitleLot Number
      Anti-phospho-CREB (Ser133), clone 10E9 - 2441887 2441887
      Anti-phospho-CREB (Ser133), -2507078 2507078
      Anti-phospho-CREB (Ser133), -2528751 2528751
      Anti-phospho-CREB (Ser133), -2549035 2549035
      Anti-phospho-CREB (Ser133), -2552364 2552364
      Anti-phospho-CREB (Ser133), -2605461 2605461
      Anti-phospho-CREB (Ser133), -2697627 2697627
      Anti-phospho-CREB (Ser133), -2709003 2709003
      Anti-phospho-CREB (Ser133), -2728407 2728407
      Anti-phospho-CREB (Ser133), clone 10E9 3079126

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies